Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IHC | See 2 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
12380-1-AP targets HDGFRP3 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3046 Product name: Recombinant human HDGFRP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-203 aa of BC015483 Sequence: MARPRPREYKAGDLVFAKMKGYPHWPARIDELPEGAVKPPANKYPIFFFGTHETAFLGPKDLFPYKEYKDKFGKSNKRKGFNEGLWEIENNPGVKFTGYQAIQQQSSSETEGEGGNTADASSEEEGDRVEEDGKGKRKNEKAGSKRKKSYTSKKSSKQSRKSPGDEDDKDCKEEENKSSSEGGDAGNDTRNTTSDLQKTSEGT 相同性解析による交差性が予測される生物種 |
| Full Name | hepatoma-derived growth factor, related protein 3 |
| Calculated molecular weight | 203 aa, 23 kDa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | BC015483 |
| Gene Symbol | HDGFRP3 |
| Gene ID (NCBI) | 50810 |
| RRID | AB_2117222 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y3E1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HDGFRP3, also named as Hepatoma-derived growth factor-related protein 3 or HDGF2, is a 203 amino acid protein, which contains 1 PWWP domain and belongs to the HDGF family. HDGFRP3 detected in testis, heart, spinal cord and brain. HDGFRP3 as a nuclear potein enhances DNA synthesis and may play a role in cell proliferation. HDGFRP3 is a 30 kDa protein for phosphor modification.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HDGFRP3 antibody 12380-1-AP | Download protocol |
| IHC protocol for HDGFRP3 antibody 12380-1-AP | Download protocol |
| IP protocol for HDGFRP3 antibody 12380-1-AP | Download protocol |
| WB protocol for HDGFRP3 antibody 12380-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Immunol A robust CD8+ T cell-related classifier for predicting the prognosis and efficacy of immunotherapy in stage III lung adenocarcinoma | ||
Biochem Biophys Res Commun Depletion of hepatoma-derived growth factor-related protein-3 induces apoptotic sensitization of radioresistant A549 cells via reactive oxygen species-dependent p53 activation.
| ||
Biochem Biophys Res Commun Knockdown of hepatoma-derived growth factor-related protein-3 induces apoptosis of H1299 cells via ROS-dependent and p53-independent NF-κB activation. | ||
Cancer Sci A novel function of HRP-3 in regulating cell cycle progression via the HDAC-E2F1-Cyclin E pathway in lung cancer
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