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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HDDC2 Monoclonal antibody

HDDC2 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2E1B2

Cat no : 68391-1-Ig

Synonyms

C6orf74, CGI 130, dJ167O5.2, HD domain containing 2, HD domain containing protein 2, HDDC2, NS5ATP2

Validation Data Gallery

Applications:
  • WB analysis using 68391-1-Ig

    WB analysis using 68391-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68391-1-Ig (HDDC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of Saos-2 using 68391-1-Ig

    IF Staining of Saos-2 using 68391-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using HDDC2 antibody (68391-1-Ig, Clone: 2E1B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, MCF-7 cells
Positive IF detected inSaos-2 cells
Planning an IHC experiment? We recommend our IHCeasy HDDC2 Ready-To-Use IHC Kit. HDDC2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

68391-1-Ig targets HDDC2 in WB, IF, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HDDC2 fusion protein Ag33258 相同性解析による交差性が予測される生物種
Full Name HD domain containing 2
Calculated molecular weight 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC003357
Gene symbol HDDC2
Gene ID (NCBI) 51020
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HDDC2, also named as C6orf74, plays a role in the maintenance of pluripotency in human ES and iPS cells. HDDC2 is a nuclear‐encoded but mitochondrially localized protein. It has been reported as associated with oxidative metabolism.

Protocols

Product Specific Protocols
WB protocol for HDDC2 antibody 68391-1-IgDownload protocol
IF protocol for HDDC2 antibody 68391-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols