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HBP1 Polyclonal antibody
HBP1 Polyclonal Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC, IF, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 11746-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human brain tissue, SGC-7901 cells |
| Positive IP detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 8 publications below |
| WB | See 13 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
| ChIP | See 1 publications below |
Product Information
11746-1-AP targets HBP1 in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HBP1 fusion protein Ag2342 相同性解析による交差性が予測される生物種 |
| Full Name | HMG-box transcription factor 1 |
| Calculated molecular weight | 514 aa, 58 kDa |
| Observed molecular weight | 58 kDa |
| GenBank accession number | BC022329 |
| Gene symbol | HBP1 |
| Gene ID (NCBI) | 26959 |
| RRID | AB_2247970 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HMG-box protein 1 (HBP1) is a transcriptional repressor, and has a role in cell cycle progression and tumor suppression. Also it's a repressor of the cyclin D1 gene and inhibits the Wnt signaling pathway. Interaction with RB1 enhances it's binding to the H1F0 promoter . Disrupts the interaction between DNA and TCF4. This is a rabbit polyclonal antibody raised against part of C-terminus of HBP1 of human origin.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HBP1 antibody 11746-1-AP | Download protocol |
| IP protocol for HBP1 antibody 11746-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Cell MYC through miR-17-92 suppresses specific target genes to maintain survival, autonomous proliferation, and a neoplastic state. | ||
Oncogene miR-29c&b2 encourage extramedullary infiltration resulting in the poor prognosis of acute myeloid leukemia.
| ||
Oncogene CRISPR interference and activation of the microRNA-3662-HBP1 axis control progression of triple-negative breast cancer. | ||
EMBO Rep The human GID complex engages two independent modules for substrate recruitment. | ||
J Cell Physiol Transcription factor HMG box-containing protein 1 (HBP1) modulates mitotic clonal expansion (MCE) during adipocyte differentiation.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH S (Verified Customer) (11-23-2021) | This antibody gave a strong one band signal.
|
