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HADHA Polyclonal antibody

Name: HADHA Polyclonal antibody
Brand: Proteintech
SKU: 10758-1-AP

HADHA Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10758-1-AP

Synonyms

78 kDa gastrin binding protein, ECHA, HADH, HADHA, LCHAD, MTPA, TP ALPHA

Validation Data Gallery

Applications:

AllWBIPIHCIFFC

WB analysis of HeLa cells using 10758-1-AP
WB result of HADHA antibody (10758-1-AP, 1:4000) with si-control and si-HADHA transfected HeLa cells.
WB analysis using 10758-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10758-1-AP (HADHA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 10758-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10758-1-AP (HADHA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10758-1-AP
IP result of anti-HADHA (IP:10758-1-AP, 4ug; Detection:10758-1-AP 1:10000) with HeLa cells lysate 1080 ug.
IHC staining of human heart using 10758-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10758-1-AP (HADHA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with HADHA antibody included
IHC staining of human heart using 10758-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10758-1-AP (HADHA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with HADHA antibody included
IHC staining of human ovary using 10758-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 10758-1-AP (HADHA antibody) at dilution of 1:50 (under 40x lens).
Ready-to-use IHC kits available with HADHA antibody included
IHC staining of human colon cancer using 10758-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10758-1-AP (HADHA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with HADHA antibody included
IHC staining of human colon cancer using 10758-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10758-1-AP (HADHA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with HADHA antibody included
IHC staining of mouse heart using 10758-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10758-1-AP (HADHA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with HADHA antibody included
IHC staining of mouse heart using 10758-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10758-1-AP (HADHA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with HADHA antibody included
IF Staining of HeLa cells using 10758-1-AP
IF result of HADHA antibody (10758-1-AP, 1:50) with si-control and si-HADHA transfected HeLa cells.
IF Staining of HeLa using 10758-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10758-1-AP (HADHA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 10758-1-AP
Immunofluorescent analysis of HepG2 cells, using HADHA antibody 10758-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
FC experiment of HepG2 using 10758-1-AP
1X10^6 HepG2 cells were stained with 0.2ug HADHA antibody (10758-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Cat No. 10758-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Jurkat cells, HEK-293 cells, HeLa cells, mouse liver tissue, rat liver tissue, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human heart tissue, human ovary tissue, human colon cancer tissue, mouse heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HeLa cells, HepG2 cells
Positive FC detected in	HepG2 cells

Planning an IHC experiment? We recommend our IHCeasy HADHA Ready-To-Use IHC Kit. HADHA primary antibody included.

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:50-1:500
Flow Cytometry (FC)	FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Published Applications

WB	See 21 publications below
IHC	See 1 publications below
IF	See 1 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

10758-1-AP targets HADHA in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HADHA fusion protein Ag1211 相同性解析による交差性が予測される生物種
Full Name	hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit
Calculated molecular weight	83 kDa
Observed molecular weight	70-79 kDa
GenBank accession number	BC009235
Gene symbol	HADHA
Gene ID (NCBI)	3030
RRID	AB_2115593
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HADHA(Trifunctional enzyme subunit alpha, mitochondrial) is also named as HADH,78 kDa gastrin-binding protein.It belongs to the enoyl-CoA hydratase/isomerase family in the N-terminal section and the 3-hydroxyacyl-CoA dehydrogenase family in the central section.It harbors the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities.Defects in HADHA are a cause of trifunctional protein deficiency (TFP deficiency) and long-chain 3-hydroxyl-CoA dehydrogenase deficiency (LCHAD deficiency) and maternal acute fatty liver of pregnancy (AFLP).

Protocols

Product Specific Protocols
WB protocol for HADHA antibody 10758-1-AP	Download protocol
IHC protocol for HADHA antibody 10758-1-AP	Download protocol
IF protocol for HADHA antibody 10758-1-AP	Download protocol
IP protocol for HADHA antibody 10758-1-AP	Download protocol
FC protocol for HADHA antibody 10758-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Mitochondrial Sirtuin Network Reveals Dynamic SIRT3-Dependent Deacetylation in Response to Membrane Depolarization. Authors - Wen Yang View Article
human	WB	Cell Metab DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling. Authors - Ricarda Richter-Dennerlein View Article
pig	WB	Sci Total Environ Protein lysine acetylation played an important role in NH3-induced AEC2 damage and pulmonary fibrosis in piglets Authors - Xiaotong Wang View Article
mouse	WB	Cell Rep Feedback repression of PPARα signaling by Let-7 microRNA. Authors - Tomoki Yagai View Article
mouse	WB	Oxid Med Cell Longev Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation. Authors - Wei Wang View Article
rat	WB	Biochim Biophys Acta Succinate accumulation impairs cardiac pyruvate dehydrogenase activity through GRP91-dependent and independent signaling pathways: therapeutic effects of ginsenoside Rb1. Authors - Jia Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HADHA Polyclonal antibody

HADHA Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis of HeLa cells using 10758-1-AP

WB analysis using 10758-1-AP

WB analysis using 10758-1-AP

IP experiment of HeLa using 10758-1-AP

IHC staining of human heart using 10758-1-AP

IHC staining of human heart using 10758-1-AP

IHC staining of human ovary using 10758-1-AP

IHC staining of human colon cancer using 10758-1-AP

IHC staining of human colon cancer using 10758-1-AP

IHC staining of mouse heart using 10758-1-AP

IHC staining of mouse heart using 10758-1-AP

IF Staining of HeLa cells using 10758-1-AP

IF Staining of HeLa using 10758-1-AP

IF Staining of HepG2 using 10758-1-AP

FC experiment of HepG2 using 10758-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Mitochondrial Sirtuin Network Reveals Dynamic SIRT3-Dependent Deacetylation in Response to Membrane Depolarization.

DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling.

Protein lysine acetylation played an important role in NH3-induced AEC2 damage and pulmonary fibrosis in piglets

Feedback repression of PPARα signaling by Let-7 microRNA.

Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.

Succinate accumulation impairs cardiac pyruvate dehydrogenase activity through GRP91-dependent and independent signaling pathways: therapeutic effects of ginsenoside Rb1.