Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 19828-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of A2780 using 19828-1-AP
WB result of HADH antibody (19828-1-AP; 1:6000; room temperature for 1.5 hours) with wild-type and HADH knockout A2780 cells.
WB analysis of mouse liver using 19828-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 19828-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 19828-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 19828-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 19828-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 19828-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 19828-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 19828-1-AP
IP result of anti-HADH (IP:19828-1-AP, 3ug; Detection:19828-1-AP 1:700) with HepG2 cells lysate 400ug.
IHC staining of human breast cancer using 19828-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19828-1-AP (HADH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 19828-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19828-1-AP (HADH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 19828-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19828-1-AP (HADH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human hepatocirrhosis using 19828-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19828-1-AP (HADH antibody) at dilution of 1:400 (under 10x lens).
IHC staining of human hepatocirrhosis using 19828-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19828-1-AP (HADH antibody) at dilution of 1:400 (under 40x lens).
IHC staining of human hepatocirrhosis using 19828-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19828-1-AP (HADH antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human hepatocirrhosis using 19828-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19828-1-AP (HADH antibody) at dilution of 1:200 (under 40x lens).
IF Staining of HepG2 using 19828-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 19828-1-AP (HADH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

HADH Polyclonal antibody

HADH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 19828-1-AP

Publications(27)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

HCDH, HADHSC, HADH1, HAD1, HAD

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (27)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, A2780 cells, Jurkat cells, mouse heart tissue, mouse kidney tissue, mouse liver tissue, mouse pancreas tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human breast cancer tissue, human liver cancer tissue, human hepatocirrhosis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 25 publications below
IHC	See 2 publications below
IF	See 1 publications below
IP	See 2 publications below

Product Information

19828-1-AP targets HADH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag13928 Product name: Recombinant human HADH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-314 aa of BC000306 Sequence: MAFVTRQFMRSVSSSSTASASAKKIIVKHVTVIGGGLMGAGIAQVAAATGHTVVLVDQTEDILAKSKKGIEESLRKVAKKKFAENPKAGDEFVEKTLSTIATSTDAASVVHSTDLVVEAIVENLKVKNELFKRLDKFAAEHTIFASNTSSLQITSIANATTRQDRFAGLHFFNPVPVMKLVEVIKTPMTSQKTFESLVDFSKALGKHPVSCKDTPGFIVNRLLVPYLMEAIRLYERGDASKEDIDTAMKLGAGYPMGPFELLDYVGLDTTKFIVDGWHEMDAENPLHQPSPSLNKLVAENKFGKKTGEGFYKYK 相同性解析による交差性が予測される生物種
Full Name	hydroxyacyl-Coenzyme A dehydrogenase
Calculated molecular weight	314 aa, 34 kDa
Observed molecular weight	30-34 kDa
GenBank accession number	BC000306
Gene Symbol	HADH
Gene ID (NCBI)	3033
RRID	AB_10667408
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q16836
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HADH(Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial) is also named as HAD, HADHSC, SCHAD and belongs to the 3-hydroxyacyl-CoA dehydrogenase family. It catalyzes the reversible dehydrogenation of 3-hydroxyacyl-CoAs to their corresponding 3-ketoacyl-CoAs with concomitant reduction of NAD to NADH and exerts it highest activity toward 3-hydroxydecanoyl-CoA. . Human HADH encodes a deduced 314-amino acid protein composed of a 12-residue mitochondrial import signal peptide and a 302-residue mature HADH protein with a calculated molecular mass of 34.3 kD.

Protocols

Product Specific Protocols
IF protocol for HADH antibody 19828-1-AP	Download protocol
IHC protocol for HADH antibody 19828-1-AP	Download protocol
IP protocol for HADH antibody 19828-1-AP	Download protocol
WB protocol for HADH antibody 19828-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Metab Pyruvate-supported flux through medium-chain ketothiolase promotes mitochondrial lipid tolerance in cardiac and skeletal muscles Authors - Timothy R Koves View Article
human	IP, WB	Hepatology Disruption of the histidine triad nucleotide-binding hint2 gene in mice affects glycemic control and mitochondrial function. Authors - Martin Juliette J View Article
mouse	WB	EMBO Rep SCFJFK is functionally linked to obesity and metabolic syndrome. Authors - Lin He View Article
rat	WB	EBioMedicine PPARγ maintains the metabolic heterogeneity and homeostasis of renal tubules. Authors - Zhongshi Lyu View Article
human	WB	Front Oncol Multi-Omics Analysis of Fatty Acid Metabolism in Thyroid Carcinoma. Authors - Jinghui Lu View Article
mouse	WB	Sci Rep Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice. Authors - Lei Wu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 19828-1-AP

WB analysis of A2780 using 19828-1-AP

WB analysis of mouse liver using 19828-1-AP

WB analysis of mouse pancreas using 19828-1-AP

WB analysis of HepG2 using 19828-1-AP

WB analysis of mouse skeletal muscle using 19828-1-AP

WB analysis of Jurkat using 19828-1-AP

WB analysis of mouse heart using 19828-1-AP

WB analysis of mouse kidney using 19828-1-AP

IP experiment of HepG2 using 19828-1-AP

IHC staining of human breast cancer using 19828-1-AP

IHC staining of human liver cancer using 19828-1-AP

IHC staining of human liver cancer using 19828-1-AP

IHC staining of human hepatocirrhosis using 19828-1-AP

IHC staining of human hepatocirrhosis using 19828-1-AP

IHC staining of human hepatocirrhosis using 19828-1-AP

IHC staining of human hepatocirrhosis using 19828-1-AP

IF Staining of HepG2 using 19828-1-AP

HADH Polyclonal antibody

HADH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Pyruvate-supported flux through medium-chain ketothiolase promotes mitochondrial lipid tolerance in cardiac and skeletal muscles

Disruption of the histidine triad nucleotide-binding hint2 gene in mice affects glycemic control and mitochondrial function.

SCFJFK is functionally linked to obesity and metabolic syndrome.

PPARγ maintains the metabolic heterogeneity and homeostasis of renal tubules.

Multi-Omics Analysis of Fatty Acid Metabolism in Thyroid Carcinoma.

Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice.