HA tag Polyclonal antibody

HA tag Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein and More (8)

Applications

WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 51064-2-AP

Synonyms

HA tag, YPYDVPDYA


"HA tag Antibodies" Comparison

View side-by-side comparison of HA tag antibodies from other vendors to find the one that best suits your research needs.
Applications:
  • WB analysis of recombinant protein using 51064-2-AP

    WB analysis of recombinant protein using 51064-2-AP

    Western blot of HA-tagged fusion protein with anti-HA-tag (51064-2-AP) at various dilutions.

  • WB analysis of Transfected HEK-293 using 51064-2-AP

    WB analysis of Transfected HEK-293 using 51064-2-AP

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 51064-2-AP (HA tag antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of Transfected HEK-293 using 51064-2-AP

    IP experiment of Transfected HEK-293 using 51064-2-AP

    IP Result of anti-HA tag (IP:51064-2-AP, 3ug; Detection:51064-2-AP 1:2000) with Transfected HEK-293 cells lysate 500ug.

  • IF Staining of Transfected HEK-293 using 51064-2-AP

    IF Staining of Transfected HEK-293 using 51064-2-AP

    Immunofluorescent analysis of Transfected HEK-293 cells using 51064-2-AP (HA tag antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of Transfected HEK-293 using 51064-2-AP

    IF Staining of Transfected HEK-293 using 51064-2-AP

    Immunofluorescent analysis of Transfected HEK-293 cells using 51064-2-AP (HA tag antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of Transfected HEK-293 using 51064-2-AP

    IF Staining of Transfected HEK-293 using 51064-2-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using HA tag antibody (51064-2-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).


Tested Applications

Positive WB detected inrecombinant protein, Transfected HEK-293 cells
Positive IP detected inTransfected HEK-293 cells
Positive IF detected inTransfected HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

51064-2-AP targets HA tag in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Cited Reactivity human, chicken, rat, mouse, monkey, zebrafish, duck, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name HA tag
Calculated molecular weight 1 kDa
Gene symbol
Gene ID (NCBI)
RRIDAB_11042321
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.

Protocols

Product Specific Protocols
WB protocol for HA tag antibody 51064-2-APDownload protocol
IF protocol for HA tag antibody 51064-2-APDownload protocol
IP protocol for HA tag antibody 51064-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Signal Transduct Target Ther

FBXW7β loss-of-function enhances FASN-mediated lipogenesis and promotes colorectal cancer growth

Authors - Wenxia Wei
WB

Cell

RetroCHMP3 blocks budding of enveloped viruses without blocking cytokinesis.

Authors - Lara Rheinemann
WB

Cell

Glycogen accumulation and phase separation drives liver tumor initiation.

Authors - Qingxu Liu
humanWB

Gastroenterology

PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis

Authors - Tian-Yi Jiang
WB

Cell

The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest.

Authors - Jakob Trendel
WB

Nat Genet

Pathogenic SPTBN1 variants cause an autosomal dominant neurodevelopmental syndrome.

Authors - Margot A Cousin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

manohar (Verified Customer) (07-10-2023)

Used Nitrocellulose membrane with 5% milk as blocking for 1 hr, 1% milk used for antibody dilution and kept for overnight incubation

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293, Neuronal stem cells
FH

X (Verified Customer) (07-11-2022)

Good for both WB and IF in transfected cells

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Transfected N2A cells
FH

Victor (Verified Customer) (06-13-2022)

I'm using the antibody for Western blotting tissue culture samples and it works well for my needs for the price. I've been using a 1:2000 dilution which gives me decent bands via Western blot and analyzed using a fluorescent anti-rabbit secondary. The bands could be a little brighter, but this antibody is worth it for the sale price. It's stored in glycerol, so it saves you the trouble of aliquoting and it can be kept at -20 which is nice.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
FH

Nick (Verified Customer) (04-20-2022)

The antibody works well and performs consistently in IP and WB

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
FH

Deyong (Verified Customer) (03-11-2022)

Overall it worked good in the HEK293T cells with protein overexpression, but there were multi non specific bands below.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/3000
  • Cell Tissue Type: HEK293T cells
FH

Stephanie (Verified Customer) (01-28-2022)

Antibody easy to use, right recommended dilution. No background observed and clean bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1000
FH

S (Verified Customer) (11-23-2021)

Overall, the antibody worked well, but there was a strong band in my control (negative) lane.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HEK293T
HA tag Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:51064-2-AP)
FH

K (Verified Customer) (07-15-2021)

This ab recognized the HA tag-ed proteins, but also got a lot of non-specific bands, dilution used were 1:2000

  • Applications: Western Blot
  • Cell Tissue Type: 293T
FH

Fen (Verified Customer) (03-06-2020)

it works great for both western blot and IP

  • Applications: Western Blot, Immunoprecipitation,
  • Cell Tissue Type: B cells
FH

Chun (Verified Customer) (12-05-2019)

An excellent antibody

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5,000
  • Cell Tissue Type: HEK293 cells
FH

Nikhil (Verified Customer) (10-16-2019)

HA tagged VprBP protein was expressed in H1299 cells. Blue: DAPI; Green:HA-VprBP

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: H1299
HA tag Antibody Immunofluorescence, validation (1:100 dilution) in H1299 (Cat no:51064-2-AP)
FH

Phuong (Verified Customer) (06-19-2019)

HA tag polyclonal antibody works very well. Gives very clean and strong signal.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: 293T