HA Tag Monoclonal antibody
HA Tag Monoclonal Antibody for FC, IP, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
recombinant protein and More (4)
Applications
WB, PLA, IP, IF, FC, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
1F5C6
Cat no : 66006-2-Ig
Synonyms
"HA Tag Antibodies" Comparison
View side-by-side comparison of HA Tag antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | Transfected HEK-293 cells |
| Positive IP detected in | Transfected HEK-293 cells |
| Positive FC detected in | Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:10000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 47 publications below |
| IF | See 10 publications below |
| IP | See 17 publications below |
| ELISA | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
66006-2-Ig targets HA Tag in WB, PLA, IP, IF, FC, CoIP, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | Duck, human, mouse, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | HA Tag |
| Gene symbol | |
| Gene ID (NCBI) | |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HA Tag antibody 66006-2-Ig | Download protocol |
| IP protocol for HA Tag antibody 66006-2-Ig | Download protocol |
| FC protocol for HA Tag antibody 66006-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biosci Rep STK33/ERK2 signal pathway contribute the tumorigenesis of colorectal cancer HCT15 cells. | ||
J Virol ZAP, a CCCH-Type Zinc Finger Protein inhibits PRRSV replication and interacts with viral Nsp9. | ||
J Virol Chicken organic anion transporting polypeptide 1A2, a novel avian hepatitis E virus (HEV) ORF2-interacting protein, is involved in avian HEV infection. | ||
Mol Oncol FAM175B promotes apoptosis by inhibiting ATF4 ubiquitination in esophageal squamous cell carcinoma. | ||
Cell Cycle FAIM-S functions as a negative regulator of NF-κB pathway and blocks cell cycle progression in NSCLC cells. |
