HA Tag Monoclonal antibody
HA Tag Monoclonal Antibody for FC, IP, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
recombinant protein and More (4)
Applications
WB, PLA, IP, IF, FC, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
1F5C6
Cat no : 66006-2-Ig
Synonyms
"HA Tag Antibodies" Comparison
View side-by-side comparison of HA Tag antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
Positive WB detected in | Transfected HEK-293 cells |
Positive IP detected in | Transfected HEK-293 cells |
Positive FC detected in | Transfected HEK-293 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:10000-1:100000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 47 publications below |
IF | See 10 publications below |
IP | See 17 publications below |
ELISA | See 1 publications below |
CoIP | See 1 publications below |
Product Information
66006-2-Ig targets HA Tag in WB, PLA, IP, IF, FC, CoIP, ELISA applications and shows reactivity with recombinant protein samples.
Tested Reactivity | recombinant protein |
Cited Reactivity | Duck, human, mouse, pig |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | HA Tag |
Gene symbol | |
Gene ID (NCBI) | |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for HA Tag antibody 66006-2-Ig | Download protocol |
IP protocol for HA Tag antibody 66006-2-Ig | Download protocol |
FC protocol for HA Tag antibody 66006-2-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biosci Rep STK33/ERK2 signal pathway contribute the tumorigenesis of colorectal cancer HCT15 cells. | ||
J Virol ZAP, a CCCH-Type Zinc Finger Protein inhibits PRRSV replication and interacts with viral Nsp9. | ||
J Virol Chicken organic anion transporting polypeptide 1A2, a novel avian hepatitis E virus (HEV) ORF2-interacting protein, is involved in avian HEV infection. | ||
Mol Oncol FAM175B promotes apoptosis by inhibiting ATF4 ubiquitination in esophageal squamous cell carcinoma. | ||
Cell Cycle FAIM-S functions as a negative regulator of NF-κB pathway and blocks cell cycle progression in NSCLC cells. |