HA Tag Monoclonal antibody

HA Tag Monoclonal Antibody for FC, IP, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein and More (4)

Applications

WB, PLA, IP, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1F5C6

Cat no : 66006-2-Ig

Synonyms

HA Tag, YPYDVPDYA


"HA Tag Antibodies" Comparison

View side-by-side comparison of HA Tag antibodies from other vendors to find the one that best suits your research needs.
Applications:
  • WB analysis of HEK-293 using 66006-2-Ig

    WB analysis of HEK-293 using 66006-2-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66006-2-Ig (HA Tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IP experiment of Transfected HEK-293 using 66006-2-Ig

    IP experiment of Transfected HEK-293 using 66006-2-Ig

    IP result of anti-HA Tag (IP:66006-2-Ig, 5ug; Detection:66006-2-Ig 1:2000) with Transfected HEK-293 cells lysate 1600 ug.

  • FC experiment of Transfected HEK-293 using 66006-2-Ig

    FC experiment of Transfected HEK-293 using 66006-2-Ig

    1X10^6 Transfected HEK-293 cells were stained with 0.2 ug Anti- HA Tag (66006-2-Ig, Clone:1F5C6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

  • FC experiment of Transfected HEK-293 using 66006-2-Ig

    FC experiment of Transfected HEK-293 using 66006-2-Ig

    1X10^6 Transfected HEK-293 cells were stained with 0.2 ug Anti-N/A HA Tag (66006-2-Ig, Clone:1F5C6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

  • FC experiment of Transfected HEK-293 using 66006-2-Ig

    FC experiment of Transfected HEK-293 using 66006-2-Ig

    1X10^6 Transfected HEK-293 cells were stained with 0.2 ug Anti-N/A HA Tag (66006-2-Ig, Clone:1F5C6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.


Tested Applications

Positive WB detected inTransfected HEK-293 cells
Positive IP detected inTransfected HEK-293 cells
Positive FC detected inTransfected HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:10000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Sample-dependent, check data in validation data gallery

Product Information

66006-2-Ig targets HA Tag in WB, PLA, IP, IF, FC, CoIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Cited Reactivity Duck, human, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name HA Tag
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.

Protocols

Product Specific Protocols
WB protocol for HA Tag antibody 66006-2-IgDownload protocol
IP protocol for HA Tag antibody 66006-2-IgDownload protocol
FC protocol for HA Tag antibody 66006-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Biosci Rep

STK33/ERK2 signal pathway contribute the tumorigenesis of colorectal cancer HCT15 cells.

Authors - Shengjun Zhang

J Virol

ZAP, a CCCH-Type Zinc Finger Protein inhibits PRRSV replication and interacts with viral Nsp9.

Authors - Yongxiang Zhao

J Virol

Chicken organic anion transporting polypeptide 1A2, a novel avian hepatitis E virus (HEV) ORF2-interacting protein, is involved in avian HEV infection.

Authors - Huixia Li

Mol Oncol

FAM175B promotes apoptosis by inhibiting ATF4 ubiquitination in esophageal squamous cell carcinoma.

Authors - Yu Zhao

Cell Death Dis

A Cdh1-FoxM1-Apc axis controls muscle development and regeneration.

Authors - Zhe Chen
WB

Cell Cycle

FAIM-S functions as a negative regulator of NF-κB pathway and blocks cell cycle progression in NSCLC cells.

Authors - Pengcheng Wang