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HA Tag 抗体 58 文献

Mouse Monoclonal| カタログ番号: 66006-2-Ig |クローン番号: 1F5C6

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適用アプリケーション

アプリケーション:
WB, PLA, IP, IF, FC
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Images See all

HA Tag Antibody WB HEK-293 cells 66006-2-Ig

HEK-293 cells were subjected to SDS PAGE followed by western blot with 66006-2-Ig (HA Tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

HA Tag Antibody IP Transfected HEK-293 cells 66006-2-Ig

IP result of anti-HA Tag (IP:66006-2-Ig, 5ug; Detection:66006-2-Ig 1:2000) with Transfected HEK-293 cells lysate 1600 ug.

HA Tag Antibody FC Transfected HEK-293 cells 66006-2-Ig

1X10^6 Transfected HEK-293 cells were stained with 0.2 ug Anti- HA Tag (66006-2-Ig, Clone:1F5C6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

種特異性:
recombinant protein, Duck, human, mouse, pig
WB ポジティブ:
Transfected HEK-293 cells
IP ポジティブ:
Transfected HEK-293 cells
FC ポジティブ:
Transfected HEK-293 cells
推奨希釈倍率:
WB : 1:10000-1:100000
IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB

Protocols

Product-specific protocols

Standard Protocols

製品情報

由来:
Mouse
精製方法:
Protein A purification
アイソタイプ:
IgG1
バッファー/保存方法:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

ターゲット情報

免疫原:
Peptide
正式名:
HA Tag
計算分子量:
検出分子量:
GenBank accession number:
Gene ID (NCBI):
遺伝子シンボル:
別名:
HA Tag, YPYDVPDYA

背景説明

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.


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