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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using GH1 Polyclonal antibody (27079-1-AP)

    WB analysis of human placenta using 27079-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 27079-1-AP (GH1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pituitary adenoma tissue using GH1 Polyclonal antibody (27079-1-AP)

    IHC staining of human pituitary adenoma using 27079-1-AP

    Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 27079-1-AP (GH1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pituitary adenoma tissue using GH1 Polyclonal antibody (27079-1-AP)

    IHC staining of human pituitary adenoma using 27079-1-AP

    Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 27079-1-AP (GH1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using GH1 Polyclonal antibody (27079-1-AP)

    IHC staining of human placenta using 27079-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27079-1-AP (GH1 antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using GH1 Polyclonal antibody (27079-1-AP)

    IHC staining of human placenta using 27079-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27079-1-AP (GH1 antibody at dilution of 1:200 (under 40x lens).

Growth Hormone Polyclonal antibody

Growth Hormone Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27079-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

GH, GH1, growth hormone 1, GH-N, GH 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman pituitary adenoma tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27079-1-AP targets Growth Hormone in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag22841

Product name: Recombinant human GH1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 18-98 aa of BC075012

Sequence: LPWLQEGSAFPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSN

相同性解析による交差性が予測される生物種
Full Name GH1
Calculated molecular weight 217 aa, 25 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC075012
Gene Symbol GH1
Gene ID (NCBI) 2688
RRIDAB_2880745
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP01241
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GH1, also named as GH or GH-N, belongs to the somatotropin/prolactin family. GH1 plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.

Protocols

Product Specific Protocols
WB protocol for Growth Hormone antibody 27079-1-APDownload protocol
IHC protocol for Growth Hormone antibody 27079-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols