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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated Glucocorticoid receptor Monoclonal antibody

Glucocorticoid receptor Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1D9A9

Cat no : CL594-66904

Synonyms

GCCR, GCR, Glucocorticoid receptor, GR, GRL, NR3C1

Validation Data Gallery

Applications:
  • IF Staining of mouse brain using CL594-66904

    IF Staining of mouse brain using CL594-66904

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Glucocorticoid receptor antibody (CL594-66904, Clone: 1D9A9 ) at dilution of 1:200.

  • FC experiment of HeLa using CL594-66904

    FC experiment of HeLa using CL594-66904

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Glucocorticoid receptor (CL594-66904, Clone:1D9A9) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inmouse brain tissue
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NR3C1 Ready-To-Use IHC Kit. NR3C1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66904-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-66904 targets Glucocorticoid receptor in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Glucocorticoid receptor fusion protein Ag28431 相同性解析による交差性が予測される生物種
Full Name nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
Calculated molecular weight 86 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC015610
Gene symbol NR3C1
Gene ID (NCBI) 2908
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NR3C1 is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight [PMID:22810058].

Protocols

Product Specific Protocols
IF protocol for CL594 Glucocorticoid receptor antibody CL594-66904Download protocol
FC protocol for CL594 Glucocorticoid receptor antibody CL594-66904Download protocol
Standard Protocols
Click here to view our Standard Protocols