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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Gastrokine 1 Monoclonal antibody

Gastrokine 1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, mouse

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3C10E3

Cat no : CL488-60130

Synonyms

18 kDa antrum mucosa protein, AMP 18, AMP18, BRICD1, CA11, FOV, foveolin, gastrokine 1, GKN1, Protein CA11

Validation Data Gallery

Applications:
  • IF Staining of mouse stomach using CL488-60130

    IF Staining of mouse stomach using CL488-60130

    Immunofluorescent analysis of (4% PFA) fixed mouse stomach tissue using CoraLite® Plus 488 Gastrokine 1 antibody (CL488-60130, Clone: 3C10E3 ) at dilution of 1:200.

  • IF Staining of mouse stomach using CL488-60130

    IF Staining of mouse stomach using CL488-60130

    Immunofluorescent analysis of (4% PFA) fixed mouse stomach tissue using CoraLite® Plus 488 Gastrokine 1 antibody (CL488-60130, Clone: 3C10E3 ) at dilution of 1:200.

Tested Applications

Positive IF detected inmouse stomach tissue
For other applications, we recommend the unconjugated version of this antibody, 60130-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-60130 targets Gastrokine 1 in IF applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Gastrokine 1 fusion protein Ag6091 相同性解析による交差性が予測される生物種
Full Name gastrokine 1
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC059778
Gene symbol GKN1
Gene ID (NCBI) 56287
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Gastrokine 1 (GKN1), a stomach-specific protein also known as 18 kDa antrum mucosa protein (AMP-18) or foveolin, belongs to the gastrokine family of gastric mucus cell-secreted proteins. The human GKN1 gene has been localized in a region of chromosome 2p13 of about 6 kb and contains 6 exons. GKN1 is expressed only in normal human stomach, but is absent from gastric adenocarcinomas, gastro-esophageal adenocarcinoma cell lines, and other normal and tumor gastro-intestinal tissues. GKN1may play an important role in normal gastric function and may be a gastric tumour suppressor.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Gastrokine 1 antibody CL488-60130Download protocol
Standard Protocols
Click here to view our Standard Protocols