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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Galc Polyclonal antibody

Galc Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 51049-2-AP

Synonyms

2310068B06Rik, A930008M05Rik, AW212969, AW413532, Gacy, galactosylceramidase, Galc, twi, twitcher

Validation Data Gallery

Applications:
  • WB analysis of A375 using 51049-2-AP

    WB analysis of A375 using 51049-2-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 51049-2-AP (Galc antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of human placenta using 51049-2-AP

    WB analysis of human placenta using 51049-2-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 51049-2-AP (Galc antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 51049-2-AP

    WB analysis of mouse brain using 51049-2-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 51049-2-AP (Galc antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 51049-2-AP

    WB analysis of mouse brain using 51049-2-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 51049-2-AP (Galc antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 51049-2-AP

    WB analysis of human brain using 51049-2-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 51049-2-AP (Galc antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 51049-2-AP

    IHC staining of human prostate cancer using 51049-2-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 51049-2-AP (Galc antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inA375 cells, human brain tissue, human placenta tissue, mouse brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

51049-2-AP targets Galc in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Galc fusion protein Ag0684 相同性解析による交差性が予測される生物種
Full Name galactosylceramidase
Calculated molecular weight 77 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC086671
Gene symbol Galc
Gene ID (NCBI) 14420
RRIDAB_670391
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

GALC, also named as Galactosylceramidase , belongs to the glycosyl hydrolase 59 family. It hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. It is an enzyme with very low activity responsible for the lysosomal catabolism of galactosylceramide, a major lipid in myelin, kidney and epithelial cells of small intestine and colon. Normal GALC mRNA encodes the 80 kDa precursor, which is processed into 50 and 30 kDa subunits. (PMID:8634707 )

Protocols

Product Specific Protocols
WB protocol for Galc antibody 51049-2-APDownload protocol
IHC protocol for Galc antibody 51049-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols