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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated GYS1 Polyclonal antibody

GYS1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-10566

Synonyms

glycogen synthase 1 (muscle), GSY, GYS, GYS1

Validation Data Gallery

Applications:
  • FC experiment of HepG2 using CL647-10566

    FC experiment of HepG2 using CL647-10566

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human GYS1 (CL647-10566) (red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy GYS1 Ready-To-Use IHC Kit. GYS1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10566-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL647-10566 targets GYS1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GYS1 fusion protein Ag0857 相同性解析による交差性が予測される生物種
Full Name glycogen synthase 1 (muscle)
Calculated molecular weight 84 kDa
Observed molecular weight 84 kDa
GenBank accession numberBC007688
Gene symbol GYS1
Gene ID (NCBI) 2997
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GYS1(Glycogen [starch] synthase, muscle) is the the rate limiting enzyme of the insulin-induced glycogenesis, transferring glucose units from UDP-Glc to a glycogen primer. It catalyzes the linear addition of glucose residues to the branching structure of glycogen, providing a convenient store of glucose for times of metabolic need. This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 GYS1 antibody CL647-10566Download protocol
Standard Protocols
Click here to view our Standard Protocols