GST Tag Monoclonal antibody
GST Tag Monoclonal Antibody for IP, WB, ELISA
Cat no : 66001-2-Ig
"GST Tag Antibodies" Comparison
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|Positive WB detected in||Recombinant protein|
|Positive IP detected in||E. coli lysate|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:5000-1:20000 for WB|
|Sample-dependent, check data in validation data gallery|
66001-2-Ig targets GST Tag in WB, IP, IF, CoIP, ELISA applications and shows reactivity with Recombinant protein, Schistosoma japonicum samples.
|Tested Reactivity||Recombinant protein, Schistosoma japonicum|
|Host / Isotype||Mouse / IgG2a|
|Full Name||GST Tag|
|Calculated molecular weight||26 kDa|
|GenBank accession number||FN315687|
|Gene ID (NCBI)|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST (Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the N- or C-terminus of targeted proteins.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Lana (Verified Customer) (05-27-2022)
SDS-PAGE: 4-12% Bis-Tris gradient gel. Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4C. Blocking: SEA Block Blocking Buffer 1h, room T. Primary Ab: O/N incubation at 4C, 1:1000. Secondary Ab: IRDye 680LT Goat anti-Mouse, 1:15000. Lines of WB image: 1 – protein ladder, 2 and 3 – GST protein different amounts.
Susanne (Verified Customer) (11-18-2021)
Clear Signal, no background on E.coli lysate with expressed GST blotted on PVDF membrane
Aurelie (Verified Customer) (12-11-2018)