GST Tag Monoclonal antibody

GST Tag Monoclonal Antibody for IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Recombinant protein, Schistosoma japonicum and More (1)

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

3G12B10

Cat no : 66001-2-Ig

Synonyms

GST, GST Tag


"GST Tag Antibodies" Comparison

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Applications:
  • WB analysis of Recombinant protein using 66001-2-Ig

    WB analysis of Recombinant protein using 66001-2-Ig

    GST tagged recombinant protein (25ng/lane) was subjected to SDS-PAGE followed by western blot with anti-GST tag monoclonal antibody 66001-2-Ig at various dilutions.

  • WB analysis of Recombinant protein using 66001-2-Ig

    WB analysis of Recombinant protein using 66001-2-Ig

    GST protein (25 ng), pGEX6P-1 vector expressed in E. coli, were subjected to SDS PAGE followed by western blot with 66001-2-Ig (GST Tag antibody, 1:200,000) incubated at room temperature for 1.5 hours.

  • IP experiment of Recombinant protein using 66001-2-Ig

    IP experiment of Recombinant protein using 66001-2-Ig

    IP result of anti-GST Tag(IP:66001-2-Ig, 5ug; Detection:66001-2-Ig 1:10000) with Total E. coli lysate (with pGEX4T fused plasmid) 1360 ug.


Tested Applications

Positive WB detected inRecombinant protein
Positive IP detected inE. coli lysate

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:5000-1:20000 for WB
Sample-dependent, check data in validation data gallery

Product Information

66001-2-Ig targets GST Tag in WB, IP, IF, CoIP, ELISA applications and shows reactivity with Recombinant protein, Schistosoma japonicum samples.

Tested Reactivity Recombinant protein, Schistosoma japonicum
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Recombinant protein
Full Name GST Tag
Calculated molecular weight 26 kDa
GenBank accession numberFN315687
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST (Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the N- or C-terminus of targeted proteins.

Protocols

Product Specific Protocols
WB protocol for GST Tag antibody 66001-2-IgDownload protocol
IP protocol for GST Tag antibody 66001-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Dev Cell

FGF15 Activates Hippo Signaling to Suppress Bile Acid Metabolism and Liver Tumorigenesis.

Authors - Suyuan Ji

MBio

Nonmuscle Myosin Heavy Chain IIA Recognizes Sialic Acids on Sialylated RNA Viruses To Suppress Proinflammatory Responses via the DAP12-Syk Pathway.

Authors - Yingqi Liu

EBioMedicine

Bach1-induced suppression of angiogenesis is dependent on the BTB domain.

Authors - Li Jiang
WB,IP

Acta Pharmacol Sin

Inhibition of AXL enhances chemosensitivity of human ovarian cancer cells to cisplatin via decreasing glycolysis.

Authors - Min Tian
WB

Cell Rep

PICK1 Controls Activity-Dependent Synaptic Vesicle Cargo Retrieval.

Authors - Xuan Ling Hilary Yong
WB

Cell Rep

Regulation of NMDA receptor trafficking and gating by activity-dependent CaMKIIα phosphorylation of the GluN2A subunit.

Authors - Xuan Ling Hilary Yong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Lana (Verified Customer) (05-27-2022)

SDS-PAGE: 4-12% Bis-Tris gradient gel. Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4C. Blocking: SEA Block Blocking Buffer 1h, room T. Primary Ab: O/N incubation at 4C, 1:1000. Secondary Ab: IRDye 680LT Goat anti-Mouse, 1:15000. Lines of WB image: 1 – protein ladder, 2 and 3 – GST protein different amounts.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Lysate of Sepharose beads after GST-pull down
GST Tag Antibody Western Blot validation (1:5000 dilution) in Lysate of Sepharose beads after GST-pull down (Cat no:66001-2-Ig)
FH

Susanne (Verified Customer) (11-18-2021)

Clear Signal, no background on E.coli lysate with expressed GST blotted on PVDF membrane

  • Applications: Western Blot, Cell culture
  • Primary Antibody Dilution: 1:30000
  • Cell Tissue Type: E.coli lysate
FH

Aurelie (Verified Customer) (12-11-2018)

  • Applications: Western Blot, Immunoprecipitation,
  • Primary Antibody Dilution: 1µl for IP and 1/5000 for WB
  • Cell Tissue Type: in vitro
GST Tag Antibody Western Blot,Immunoprecipitation, validation (1µl for IP and 1/5000 for WB dilution) in in vitro (Cat no:66001-2-Ig)