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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GSDMD Recombinant antibody

GSDMD Recombinant Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2N24

Cat no : 80918-1-RR

Synonyms

DF5L, DFNA5L, FKSG10, FLJ12150, gasdermin D, GSDMD, GSDMDC1

Validation Data Gallery

Applications:
  • WB analysis using 80918-1-RR

    WB analysis using 80918-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80918-1-RR (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of RAW 264.7 using 80918-1-RR

    FC experiment of RAW 264.7 using 80918-1-RR

    1X10^6 RAW 264.7 cells were intracellularly stained with 0.4 ug Anti-Human GSDMD (80918-1-RR, Clone:2N24) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHeLa cells, A549 cells, Jurkat cells
Positive FC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

80918-1-RR targets GSDMD in WB, FC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GSDMD fusion protein Ag14757 相同性解析による交差性が予測される生物種
Full Name gasdermin D
Calculated molecular weight 484 aa, 53 kDa
Observed molecular weight50-53 kDa
GenBank accession numberBC008904
Gene symbol GSDMD
Gene ID (NCBI) 79792
RRIDAB_2918919
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GSDMD is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. GSDMD has been suggested to act as a tumor suppressor. Recently, GSDMD is identified as a new component of inflammasomes, and it is an executor of pyroptosis and required for interleukin-1β secretion. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.

Protocols

Product Specific Protocols
WB protocol for GSDMD antibody 80918-1-RRDownload protocol
FC protocol for GSDMD antibody 80918-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols