Validation Data Gallery
Tested Applications
| Positive WB detected in | Y79 cells, mouse eye tissue |
| Positive IP detected in | Y79 cells |
| Positive IHC detected in | mouse eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse eye tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
24606-1-AP targets GRK1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18063 Product name: Recombinant human GRK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 339-563 aa of BC140945 Sequence: AVELLDGQSKTKGYAGTPGFMAPELLQGEEYDFSVDYFALGVTLYEMIAARGPFRARGEKVENKELKHRIISEPVKYPDKFSQASKDFCEALLEKDPEKRLGFRDETCDKLRAHPLFKDLNWRQLEAGMLMPPFIPDSKTVYAKDIQDVGAFSTVKGVAFDKTDTEFFQEFATGNCPIPWQEEMIETGIFGELNVWRSDGQMPDDMKGISGGSSSSSKSGMCLVS 相同性解析による交差性が予測される生物種 |
| Full Name | G protein-coupled receptor kinase 1 |
| Calculated molecular weight | 563 aa, 64 kDa |
| Observed molecular weight | 64 kDa |
| GenBank accession number | BC140945 |
| Gene Symbol | GRK1 |
| Gene ID (NCBI) | 6011 |
| RRID | AB_2879635 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15835 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GRK1(G protein-coupled receptor kinase 1) is also named as RHOK, RK, GPRK1, and belongs to the AGC Ser/Thr protein kinase family. It is involved in quenching of light-induced signal transduction in the phosphoreceptor. GRK1 is a photoreceptor-specific enzyme that mediates adaptation of photoreceptors to light and protects these cells against light-induced injury.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GRK1 antibody 24606-1-AP | Download protocol |
| IHC protocol for GRK1 antibody 24606-1-AP | Download protocol |
| IP protocol for GRK1 antibody 24606-1-AP | Download protocol |
| WB protocol for GRK1 antibody 24606-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Zool Res Characterization of two novel knock-in mouse models of syndromic retinal ciliopathy carrying hypomorphic Sdccag8 mutations. | ||
Biochim Biophys Acta Mol Basis Dis Knockout of DLIC1 leads to retinal cone degeneration via disturbing Rab8 transport in zebrafish | ||
