GPX4 Monoclonal antibody
GPX4 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, mouse, rat, rabbit, pig and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
3F5G5
Cat no : 67763-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | EC109 cells, NCCIT cells, rabbit testis tissue, pig brain tissue, rat testis tisssue, mouse testis tissue, rat brain tissue, mouse brain tissue, rabbit brain tissue, Hela cells, HepG2 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, 4T1 cells, ATDC-5 cells |
| Positive IHC detected in | human liver cancer tissue, mouse testis tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1000-4000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:400-1:1600 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 35 publications below |
| IHC | See 7 publications below |
| IF | See 5 publications below |
Product Information
67763-1-Ig targets GPX4 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.
| Tested Reactivity | Human, mouse, rat, rabbit, pig |
| Cited Reactivity | human, mouse, pig, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | GPX4 fusion protein Ag30650 |
| Full Name | glutathione peroxidase 4 (phospholipid hydroperoxidase) |
| Observed molecular weight | 20-23 kDa |
| GenBank accession number | BC021567 |
| Gene symbol | GPX4 |
| Gene ID (NCBI) | 2879 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GPX4 antibody 67763-1-Ig | Download protocol |
| IHC protocol for GPX4 antibody 67763-1-Ig | Download protocol |
| IF protocol for GPX4 antibody 67763-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Discov Inhibition of DNMT-1 alleviates ferroptosis through NCOA4 mediated ferritinophagy during diabetes myocardial ischemia/reperfusion injury. | ||
Oxid Med Cell Longev Ferritinophagy-Mediated ROS Production Contributed to Proliferation Inhibition, Apoptosis, and Ferroptosis Induction in Action of Mechanism of 2-Pyridylhydrazone Dithiocarbamate Acetate. | ||
Oxid Med Cell Longev Calorie Restriction Protects against Contrast-Induced Nephropathy via SIRT1/GPX4 Activation. | ||
Oxid Med Cell Longev Cryptotanshinone Protects against PCOS-Induced Damage of Ovarian Tissue via Regulating Oxidative Stress, Mitochondrial Membrane Potential, Inflammation, and Apoptosis via Regulating Ferroptosis. | ||
Cell Death Discov Silencing of lncRNA MALAT1 facilitates erastin-induced ferroptosis in endometriosis through miR-145-5p/MUC1 signaling. | ||
Oxid Med Cell Longev miR-29a-3p in Exosomes from Heme Oxygenase-1 Modified Bone Marrow Mesenchymal Stem Cells Alleviates Steatotic Liver Ischemia-Reperfusion Injury in Rats by Suppressing Ferroptosis via Iron Responsive Element Binding Protein 2. |
