GPX4 Monoclonal antibody

GPX4 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, rabbit, pig and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3F5G5

Cat no : 67763-1-Ig

Synonyms

Glutathione peroxidase 4, GPx 4, GPX4, GSHPx 4, MCSP, PHGPx, snGPx, snPHGPx



Tested Applications

Positive WB detected inEC109 cells, NCCIT cells, rabbit testis tissue, pig brain tissue, rat testis tisssue, mouse testis tissue, rat brain tissue, mouse brain tissue, rabbit brain tissue, Hela cells, HepG2 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, 4T1 cells, ATDC-5 cells
Positive IHC detected inhuman liver cancer tissue, mouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1000-4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

67763-1-Ig targets GPX4 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Reactivity Human, mouse, rat, rabbit, pig
Cited Reactivityhuman, mouse, pig, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GPX4 fusion protein Ag30650
Full Name glutathione peroxidase 4 (phospholipid hydroperoxidase)
Observed molecular weight 20-23 kDa
GenBank accession numberBC021567
Gene symbol GPX4
Gene ID (NCBI) 2879
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.

Protocols

Product Specific Protocols
WB protocol for GPX4 antibody 67763-1-IgDownload protocol
IHC protocol for GPX4 antibody 67763-1-IgDownload protocol
IF protocol for GPX4 antibody 67763-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IF

Cell Death Discov

Inhibition of DNMT-1 alleviates ferroptosis through NCOA4 mediated ferritinophagy during diabetes myocardial ischemia/reperfusion injury.

Authors - Wenyuan Li
humanWB

Oxid Med Cell Longev

Ferritinophagy-Mediated ROS Production Contributed to Proliferation Inhibition, Apoptosis, and Ferroptosis Induction in Action of Mechanism of 2-Pyridylhydrazone Dithiocarbamate Acetate.

Authors - Longlong Li
ratWB

Oxid Med Cell Longev

Calorie Restriction Protects against Contrast-Induced Nephropathy via SIRT1/GPX4 Activation.

Authors - Dandong Fang
ratWB,IHC

Oxid Med Cell Longev

Cryptotanshinone Protects against PCOS-Induced Damage of Ovarian Tissue via Regulating Oxidative Stress, Mitochondrial Membrane Potential, Inflammation, and Apoptosis via Regulating Ferroptosis.

Authors - Honglin Liu
humanWB,IHC

Cell Death Discov

Silencing of lncRNA MALAT1 facilitates erastin-induced ferroptosis in endometriosis through miR-145-5p/MUC1 signaling.

Authors - Zongwen Liang
ratWB

Oxid Med Cell Longev

miR-29a-3p in Exosomes from Heme Oxygenase-1 Modified Bone Marrow Mesenchymal Stem Cells Alleviates Steatotic Liver Ischemia-Reperfusion Injury in Rats by Suppressing Ferroptosis via Iron Responsive Element Binding Protein 2.

Authors - Xiang Li