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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GPR161 Polyclonal antibody

GPR161 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 29328-1-AP

Synonyms

FLJ33952, G protein coupled receptor 161, G protein coupled receptor RE2, GPR161, RE2

Validation Data Gallery

Applications:
  • WB analysis using 29328-1-AP

    WB analysis using 29328-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29328-1-AP (GPR161 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of ARPE-19 using 29328-1-AP

    IF Staining of ARPE-19 using 29328-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed ARPE-19 cells using GPR161 antibody (29328-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red).

  • IF Staining of hTERT-RPE1 using 29328-1-AP

    IF Staining of hTERT-RPE1 using 29328-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using GPR161 antibody (29328-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red).

  • IF Staining of ARPE-19 using 29328-1-AP

    IF Staining of ARPE-19 using 29328-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed ARPE-19 cells using GPR161 antibody (29328-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red).

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF detected inARPE-19 cells, hTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

29328-1-AP targets GPR161 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPR161 fusion protein Ag30998 相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor 161
Calculated molecular weight 529 aa, 59 kDa
Observed molecular weight70 kDa
GenBank accession numberBC028163
Gene symbol GPR161
Gene ID (NCBI) 23432
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPR161 (also known as RE2) is an orphan G protein-coupled receptor and plays an important role in the Hh pathway. In the absence of Hh signals, GPR161 localizes to primary cilia and keeps the downstream GLI transcription factors in their repressor forms (PMID: 26305592). Ciliary localization of GPR161 requires TULP3 and the IFT-A complex. In the presence of SHH, GPR161 is removed from primary cilia and is internalized into recycling endosomes, preventing its activity and allowing activation of the Shh signaling. GPR161 has a calculated molecular mass of 59 kDa. The 70-kDa band detected by this antibody probably represents a modified version of GPR161 (PMID: 18250320).

Protocols

Product Specific Protocols
WB protocol for GPR161 antibody 29328-1-APDownload protocol
IF protocol for GPR161 antibody 29328-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols