• Featured Product
  • KD/KO Validated

GOT1 Polyclonal antibody

GOT1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 14886-1-AP

Synonyms

AATC, CAT, GIG18, GOT1, Transaminase A



Tested Applications

Positive WB detected inHepG2 cells, L02 cells, mouse brain, rat brain
Positive IP detected inmouse brain tissue
Positive IF detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14886-1-AP targets GOT1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GOT1 fusion protein Ag6671 相同性解析による交差性が予測される生物種
Full Name glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
Calculated molecular weight 46 kDa
Observed molecular weight 43-46 kDa
GenBank accession numberBC000498
Gene symbol GOT1
Gene ID (NCBI) 2805
RRIDAB_2113630
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glutamate oxaloacetate transaminase 1 (GOT1) catalyzes the reversible reaction of L-aspartate and alpha-ketoglutarate into oxaloacetate and L-glutamate and plays a key role in carbon and nitrogen metabolism. GOT1 can potentially control the intracellular levels of reactive oxygen species (ROS) through NADPH synthesis and enhances tumor growth. GOT1 expression correlates with the growth of several tumors.

Protocols

Product Specific Protocols
WB protocol for GOT1 antibody 14886-1-APDownload protocol
IF protocol for GOT1 antibody 14886-1-APDownload protocol
IP protocol for GOT1 antibody 14886-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.

Authors - Xiulei Mo
WB

Cell Metab

Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.

Authors - Qiuying Chen
mouseWB

Cell Res

Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.

Authors - Wei-Feng Cai
  • KD Validated
humanWB

Nat Commun

Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.

Authors - Yun-Qian Wang
humanWB

Nat Commun

Cooperative STAT/NF-κB signaling regulates lymphoma metabolic reprogramming and aberrant GOT2 expression.

Authors - Maren Feist
humanWB

J Exp Med

Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.

Authors - Miao Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Daniel (Verified Customer) (04-29-2019)

Strong band of the expected size. Weaker non-specific bands.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HK2 and RCC4 (human renal cells)
GOT1 Antibody Western Blot, validation (1:1000 dilution) in HK2 and RCC4 (human renal cells) (Cat no:14886-1-AP)