Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, mouse lung tissue, HeLa cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
| IF | See 3 publications below |
Product Information
17798-1-AP targets GORAB in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12002 Product name: Recombinant human GORAB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-246 aa of BC064945 Sequence: MSWAAVLAVAAARFGHFWGCRWPGPMAQGWAGFSEEELRRLKQTKDPFEPQRRLPAKKSRQQLQREKALVEQSQKLGLQDGSTSLLPEQLLSAPKQRVNVQKPPFSSPTLPSHFTLTSPVGDGQPQGIESQPKELGLENSHDGHNNVEILPPKPDCKLEKKKVELQEKSRWEVLQQEQRLMEEKNKRKKALLAKAIAERSKRTQAETMKLKRIQKELQALDDMVSADIGILRNRIDQASLDYSYAR 相同性解析による交差性が予測される生物種 |
| Full Name | golgin, RAB6-interacting |
| Calculated molecular weight | 264aa,28 kDa; 394aa,45 kDa |
| Observed molecular weight | 45-50 kDa |
| GenBank accession number | BC064945 |
| Gene Symbol | GORAB |
| Gene ID (NCBI) | 92344 |
| RRID | AB_10755145 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5T7V8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GORAB, also known as Scyl1BP1, is a protein that localizes to the trans-side of the Golgi apparatus and plays a significant role in intracellular trafficking and protein glycosylation. It is composed of a central coiled-coil region that is responsible for Golgi targeting, likely through interactions with small GTPases Rab6 and Arf5. GORAB is considered a member of the golgin family of coiled-coil Golgi proteins, which are involved in vesicle tethering. GORAB has been implicated in several cellular functions. It has been proposed to function as a transcriptional activator for neurite outgrowth, as a modulator of MDM2 ubiquitylation impacting p53 levels and apoptosis, and has been shown to play a role in centriole duplication and ciliogenesis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GORAB antibody 17798-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Invest Dermatol Gorab Is Required for Dermal Condensate Cells to Respond to Hedgehog Signals during Hair Follicle Morphogenesis.
| ||
FASEB J GORAB promotes embryonic lung maturation through antagonizing AKT phosphorylation, versican expression, and mesenchymal cell migration.
| ||
J Cell Sci Vimentin intermediate filaments provide structural stability to the mammalian Golgi complex | ||
Mol Vis Identification and functional analysis of a novel oculocerebrorenal syndrome of Lowe (OCRL) gene variant in two pedigrees with varying phenotypes including isolated congenital cataract. |
