Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 15060-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15060-1-AP (Nucleostemin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 15060-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 15060-1-AP (Nucleostemin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15060-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15060-1-AP (Nucleostemin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 15060-1-AP
IP result of anti-GNL3 (IP:15060-1-AP, 4ug; Detection:15060-1-AP 1:600) with HeLa cells lysate 4000ug.
IHC staining of human oesophagus cancer using 15060-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15060-1-AP (GNL3 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 15060-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15060-1-AP (GNL3 antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 15060-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15060-1-AP (GNL3 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 15060-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15060-1-AP (GNL3 antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 15060-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15060-1-AP (GNL3 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

GNL3 Polyclonal antibody

GNL3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15060-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

NNP47, Guanine nucleotide-binding protein-like 3, E2-induced gene 3 protein, E2IG3, E2 induced gene 3 protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, K-562 cells, HeLa cells, HepG2 cells, Raji cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human oesophagus cancer tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:800-1:3200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below

Product Information

15060-1-AP targets GNL3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7056 Product name: Recombinant human GNL3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 201-549 aa of BC001024 Sequence: TVVFRASTKPKDKGKITKRVKAKKNAAPFRSEVCFGKEGLWKLLGGFQETCSKAIRVGVIGFPNVGKSSIINSLKQEQMCNVGVSMGLTRSMQVVPLDKQITIIDSPSFIVSPLNSSSALALRSPASIEVVKPMEAASAILSQADARQVVLKYTVPGYRNSLEFFTMLAQRRGMHQKGGIPNVEGAAKLLWSEWTGASLAYYCHPPTSWTPPPYFNESIVVDMKSGFNLEELEKNNAQSIRAIKGPHLANSILFQSSGLTNGIIEEKDIHEELPKRKERKQEEREDDKDSDQETVDEEVDENSSGMFAAEETGEALSEETTAGEQSTRSFILDKIIEEDDAYDFSTDYV 相同性解析による交差性が予測される生物種
Full Name	guanine nucleotide binding protein-like 3 (nucleolar)
Calculated molecular weight	62 kDa
Observed molecular weight	62 kDa
GenBank accession number	BC001024
Gene Symbol	GNL3
Gene ID (NCBI)	26354
RRID	AB_2263303
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BVP2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for GNL3 antibody 15060-1-AP	Download protocol
IHC protocol for GNL3 antibody 15060-1-AP	Download protocol
IP protocol for GNL3 antibody 15060-1-AP	Download protocol
WB protocol for GNL3 antibody 15060-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Nanobiotechnology Targeted micelles with chemotherapeutics and gene drugs to inhibit the G1/S and G2/M mitotic cycle of prostate cancer. Authors - Yiran Zhang View Article
human	WB	Cancer Manag Res The oncogenic role of GNL3 in the progression and metastasis of osteosarcoma. Authors - Tianyou Li KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15060-1-AP

WB analysis of K-562 using 15060-1-AP

WB analysis of HeLa using 15060-1-AP

IP experiment of HeLa using 15060-1-AP

IHC staining of human oesophagus cancer using 15060-1-AP