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GNB5 Polyclonal antibody

GNB5 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11045-2-AP

Synonyms

GB5, Gbeta5, GNB5, Transducin beta chain 5



Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue
Positive IP detected inrat brain tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11045-2-AP targets GNB5 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNB5 fusion protein Ag1518 相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein (G protein), beta 5
Calculated molecular weight 39 kDa
Observed molecular weight 39-42 kDa
GenBank accession numberBC011671
Gene symbol GNB5
Gene ID (NCBI) 10681
RRIDAB_2109639
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. G proteins, which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit.

Protocols

Product Specific Protocols
WB protocol for GNB5 antibody 11045-2-APDownload protocol
IHC protocol for GNB5 antibody 11045-2-APDownload protocol
IP protocol for GNB5 antibody 11045-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncogene

The Gβ5 protein regulates sensitivity to TRAIL-induced cell death in colon carcinoma.

Authors - D Fuchs
  • KD Validated
ratWB

J Cell Mol Med

Prenatal hypoxia inhibited propionate-evoked BK channels of mesenteric artery smooth muscle cells in offspring.

Authors - Wenna Zhang
mouseWB

Neuropharmacology.

A systems genetic analysis of alcohol drinking by mice, rats and men: Influence of brain GABAergic transmission.

Authors - Saba LM
mouseWB, IF

PMC Biophys

Retinal cone photoreceptors require phosducin-like protein 1 for g protein complex assembly and signaling.

Authors - Christopher M Tracy
humanWB

FEBS J

PMID: 30719834

Authors - Sang-Min Park
  • KD Validated