Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, human brain tissue |
| Positive IP detected in | rat brain tissue |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| IF | See 1 publications below |
Product Information
11045-2-AP targets GNB5 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1518 Product name: Recombinant human GNB5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-126 aa of BC011671 Sequence: MATEGLHENETLASLKSEAESLKGKLEEERAKLHDVELHQVAERVEALGQFVMKTRRTLKGHGNKVLCMDWCKDKRRIVSSSQDGKVIVWDSFTTNKVRHCSQPRYRGFRIPAYLKVLWSSCPALS 相同性解析による交差性が予測される生物種 |
| Full Name | guanine nucleotide binding protein (G protein), beta 5 |
| Calculated molecular weight | 39 kDa |
| Observed molecular weight | 39-42 kDa |
| GenBank accession number | BC011671 |
| Gene Symbol | GNB5 |
| Gene ID (NCBI) | 10681 |
| RRID | AB_2109639 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14775 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. G proteins, which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GNB5 antibody 11045-2-AP | Download protocol |
| IP protocol for GNB5 antibody 11045-2-AP | Download protocol |
| WB protocol for GNB5 antibody 11045-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncogene The Gβ5 protein regulates sensitivity to TRAIL-induced cell death in colon carcinoma.
| ||
J Cell Mol Med Prenatal hypoxia inhibited propionate-evoked BK channels of mesenteric artery smooth muscle cells in offspring. | ||
Neuropharmacology. A systems genetic analysis of alcohol drinking by mice, rats and men: Influence of brain GABAergic transmission. | ||
PMC Biophys Retinal cone photoreceptors require phosducin-like protein 1 for g protein complex assembly and signaling. | ||
MedComm (2020) Transcriptomic analysis of rat brain response to alternating current electrical stimulation: unveiling insights via single-nucleus RNA sequencing |
