Validation Data Gallery
Tested Applications
| Positive WB detected in | rat kidney tissue, mouse kidney tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
Product Information
10864-2-AP targets GM2A in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1295 Product name: Recombinant human GM2A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-193 aa of BC009273 Sequence: MQSLMQAPLLIALGLLLAAPAQAHLKKPSQLSSFSWDNCDEGKDPAVIRSLTLEPDPIVVPGNVTLSVVGSTSVPLSSPLKVDLVLEKEVAGLWIKIPCTDYIGSCTFEHFCDVLDMLIPTGEPCPEPLRTYGLPCHCPFKEGTYSLPKSEFVVPDLELPSWLTTGNYRIESVLSSSGKRLGCIKIAASLKGI 相同性解析による交差性が予測される生物種 |
| Full Name | GM2 ganglioside activator |
| Calculated molecular weight | 22 kDa |
| Observed molecular weight | 22 kDa |
| GenBank accession number | BC009273 |
| Gene Symbol | GM2A |
| Gene ID (NCBI) | 2760 |
| RRID | AB_2110807 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P17900 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GM2A, also named as GM2-AP and SAP-3, is the large binding pocket which can accommodate several single chain phospholipids and fatty acids. GM2A also exhibits some calcium-independent phospholipase activity. It plays a key role in the degradation of ganglioside GM2 (GM2). GM2A stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for GM2A antibody 10864-2-AP | Download protocol |
| IHC protocol for GM2A antibody 10864-2-AP | Download protocol |
| IP protocol for GM2A antibody 10864-2-AP | Download protocol |
| WB protocol for GM2A antibody 10864-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Basic Res Cardiol Expression and regulation of type 2A protein phosphatases and alpha4 signalling in cardiac health and hypertrophy. | ||
Cell Struct Funct Glycosphingolipid GM2 Induces Invasiveness in Irradiation-tolerant Lung Cancer Cells.
| ||
Mol Neurodegener Elevated ganglioside GM2 activator (GM2A) in human brain tissue reduces neurite integrity and spontaneous neuronal activity | ||
