Validation Data Gallery
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Tested Applications
| Positive WB detected in | SH-SY5Y cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary tumor tissue, mouse placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 10 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
16768-1-AP targets GLT25D1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10346 Product name: Recombinant human GLT25D1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-231 aa of BC020492 Sequence: MLPGYRDPYHGRPLTKGELGCFLSHYNIWKEVVDRGLQKSLVFEDDLRFEIFFKRRLMNLMRDVEREGLDWDLIYVGRKRMQVEHPEKAVPRVRNLVEADYSYWTLAYVISLQGARKLLAAEPLSKMLPVDEFLPVMFDKHPVSEYKAHFSLRNLHAFSVEPLLIYPTHYTGDDGYVSDTETSVVWNNEHVKTDWDRAKSQKMREQQALSREAKNSDVLQSPLDSAARDEL 相同性解析による交差性が予測される生物種 |
| Full Name | glycosyltransferase 25 domain containing 1 |
| Calculated molecular weight | 231 aa, 27 kDa |
| Observed molecular weight | 72 kDa |
| GenBank accession number | BC020492 |
| Gene Symbol | GLT25D1 |
| Gene ID (NCBI) | 79709 |
| RRID | AB_2878313 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NBJ5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GLT25D1 antibody 16768-1-AP | Download protocol |
| IHC protocol for GLT25D1 antibody 16768-1-AP | Download protocol |
| IP protocol for GLT25D1 antibody 16768-1-AP | Download protocol |
| WB protocol for GLT25D1 antibody 16768-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Ann Neurol Biallelic COLGALT1 variants are associated with cerebral small vessel disease. | ||
J Mol Cell Cardiol Ascorbate starvation alters endoplasmic reticulum-resident enzymes in cardiac fibroblasts, priming them for increased procollagen secretion. | ||
PLoS Genet Cyclophilin B control of lysine post-translational modifications of skin type I collagen. | ||
Sci Rep Lysyl hydroxylase 2 mediated collagen post-translational modifications and functional outcomes | ||
Biochemistry Role of Glycosyltransferase 25 domain 1 in Type I Collagen Glycosylation and Molecular Phenotypes.
| ||
Mol Med Rep Downregulation of Glt25d1 aggravates carbon tetrachloride‑induced acute hepatic injury through activation of the TGF‑β1/Smad2 signaling pathway.
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