Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of HEK-293 using 15428-1-AP
WB result of GLA antibody (15428-1-AP, 1:600) with si-Control and si-GLA transfected HEK293 cells..
WB analysis using 15428-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 15428-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15428-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 15428-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 15428-1-AP
IP result of anti-Alpha Galactosidase A (IP:15428-1-AP, 3ug; Detection:15428-1-AP 1:1000) with HEK-293 cells lysate 1800ug.
IHC staining of human liver cancer using 15428-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15428-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15428-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver cancer using 15428-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse liver using 15428-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver using 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:50 (under 10x lens).
IHC staining of mouse liver using 15428-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver using 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver cancer using 15428-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15428-1-AP (Alpha galactosidase A antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15428-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15428-1-AP (Alpha galactosidase A antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 15428-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 15428-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

Alpha Galactosidase A Polyclonal antibody

Alpha Galactosidase A Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 15428-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Alpha D galactosidase A, Alpha galactosidase A, galactosidase, alpha

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	MCF-7 cells, HEK-293 cells, HeLa cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human liver cancer tissue, human liver tissue, mouse liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 1 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

15428-1-AP targets Alpha Galactosidase A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity	human, rat
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7609 Product name: Recombinant human GLA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 81-425 aa of BC002689 Sequence: WKDAGYEYLCIDDCWMAPQRDSEGRLQADPQRFPHGIRQLANYVHSKGLKLGIYADVGNKTCAGFPGSFGYYDIDAQTFADWGVDLLKFDGCYCDSLENLADGYKHMSLALNRTGRSIVYSCEWPLYMWPFQKPNYTEIRQYCNHWRNFADIDDSWKSIKSILDWTSFNQERIVDVAGPGGWNDPDMLVIGNFGLSWNQQVTQMALWAIMAAPLFMSNDLRHISPQAKALLQDKDVIAINQDPLGKQGYQLRQGDNFEVWERPLSGLAWAVAMINRQEIGGPRSYTIAVASLGKGVACNPACFITQLLPVKRKLGFYEWTSRLRSHINPTGTVLLQLENTMQMSL 相同性解析による交差性が予測される生物種
Full Name	galactosidase, alpha
Calculated molecular weight	49 kDa
Observed molecular weight	46-50 kDa
GenBank accession number	BC002689
Gene Symbol	GLA
Gene ID (NCBI)	2717
RRID	AB_10638002
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P06280
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GLA (Alpha-galactosidase A), also named as Melibiase or Agalsidase, belongs to the glycosyl hydrolase 27 family. It catalyzes the hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. The deficient activity of GLA can cause Fabry disease which is an X-linked inborn error of glycosphingolipid metabolis (PMID: 19287194). Enzyme replacement therapy (ERT) with GLA is currently the most effective therapeutic strategy for patients with Fabry disease (PMID: 20398385). In humans, GLA is synthesized as a 50 kDa precursor, which is further processed to a 46 kDa mature form of the protein (PMID: 9883849, 19387866). It also has a homodimer form with the molecular mass of 110 kDa (PMID: 17287429).

Protocols

Product Specific Protocols
IHC protocol for Alpha Galactosidase A antibody 15428-1-AP	Download protocol
IP protocol for Alpha Galactosidase A antibody 15428-1-AP	Download protocol
WB protocol for Alpha Galactosidase A antibody 15428-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB,IHC	J Extracell Vesicles Extracellular vesicles from recombinant cell factories improve the activity and efficacy of enzymes defective in lysosomal storage disorders. Authors - Joaquin Seras-Franzoso KO Validated View Article
human		Orphanet J Rare Dis Deep characterization of the anti-drug antibodies developed in Fabry disease patients, a prospective analysis from the French multicenter cohort FFABRY. Authors - Wladimir Mauhin View Article
rat	IF	Adv Mater Sequential Targeted Enzyme-Instructed Self-Assembly Supramolecular Nanofibers to Attenuate Intervertebral Disc Degeneration Authors - Yang Liu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HEK-293 using 15428-1-AP

WB analysis using 15428-1-AP

WB analysis of HEK-293 using 15428-1-AP

WB analysis of HeLa using 15428-1-AP

WB analysis of HEK-293 using 15428-1-AP

IP experiment of HEK-293 using 15428-1-AP

IHC staining of human liver cancer using 15428-1-AP

IHC staining of human liver cancer using 15428-1-AP

IHC staining of human liver cancer using 15428-1-AP

IHC staining of human liver cancer using 15428-1-AP

IHC staining of mouse liver using 15428-1-AP

IHC staining of mouse liver using 15428-1-AP

IHC staining of human liver cancer using 15428-1-AP

IHC staining of human liver cancer using 15428-1-AP

IHC staining of human liver using 15428-1-AP

IHC staining of human liver using 15428-1-AP

Alpha Galactosidase A Polyclonal antibody

Alpha Galactosidase A Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Extracellular vesicles from recombinant cell factories improve the activity and efficacy of enzymes defective in lysosomal storage disorders.

Deep characterization of the anti-drug antibodies developed in Fabry disease patients, a prospective analysis from the French multicenter cohort FFABRY.

Sequential Targeted Enzyme-Instructed Self-Assembly Supramolecular Nanofibers to Attenuate Intervertebral Disc Degeneration