Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of mouse brain using 14944-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14944-1-AP (GGPS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 14944-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 14944-1-AP (GGPS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 14944-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 14944-1-AP (GGPS1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of mouse bladder using 14944-1-AP
mouse bladder tissue were subjected to SDS PAGE followed by western blot with 14944-1-AP (GGPS1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 14944-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 14944-1-AP (GGPS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 14944-1-AP
IP result of anti-GGPS1 (IP:14944-1-AP, 4ug; Detection:14944-1-AP 1:500) with mouse brain tissue lysate 2640ug.
IHC staining of human kidney using 14944-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 14944-1-AP (GGPS1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 14944-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 14944-1-AP (GGPS1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human liver using 14944-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14944-1-AP (GGPS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 14944-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14944-1-AP (GGPS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 14944-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 14944-1-AP (GGPS1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 14944-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 14944-1-AP (GGPS1 antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

GGPS1 Polyclonal antibody

GGPS1 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 14944-1-AP

Publications(14)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Synonyms

GGPP synthase, GGPPS, GGPPS1, GGPPSase, GGPS1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (14)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, human placenta tissue, human testis tissue, mouse bladder tissue, human heart tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human kidney tissue, human liver tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 8 publications below
IHC	See 8 publications below
IF	See 3 publications below
FC	See 1 publications below

Product Information

14944-1-AP targets GGPS1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6745 Product name: Recombinant human GGPS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC067768 Sequence: MEKTQETVQRILLEPYKYLLQLPGKQVRTKLSQAFNHWLKVPEDKLQIIIEVTEMLHNASLLIDDIEDNSKLRRGFPVAHSIYGIPSVINSANYVYFLGLEKVLTLDHQDAVKLFTRQLLELHQGQGLDIYWRDNYTCPTEEEYKAMVLQKTGGLFGLAVGLMQLFSDYKEDLKPLLNTLGLFFQIRDDYANLHSKEYSENKSFCEDLTEGKFSFPTIHAIWSRPESTQVQNILRQRTENIDIKKYCVHYLEDVGSFEYTRNTLKELEAKAYKQIDARGGNPELVALVKHLSKMFKEENE 相同性解析による交差性が予測される生物種
Full Name	geranylgeranyl diphosphate synthase 1
Calculated molecular weight	35 kDa
Observed molecular weight	35 kDa
GenBank accession number	BC067768
Gene Symbol	GGPS1
Gene ID (NCBI)	9453
RRID	AB_2110990
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O95749
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GGPS1(Geranylgeranyl pyrophosphate synthase) belongs to the FPP/GGPP synthase family and catalyzes the synthesis of GGPP, an important molecule responsible for the C20-prenylation of protein and for the regulation of a nuclear hormone receptor. GGPP synthase puri¢ed from bovine brain is reported to be a homo-oligomer (150-195 kDa) with a molecular mass of 37.5 kDa for the monomer. This is consistent with the calculated molecular mass (35 kDa) of mouse and human GGPP synthases (PMID:10101267).

Protocols

Product Specific Protocols
IHC protocol for GGPS1 antibody 14944-1-AP	Download protocol
IP protocol for GGPS1 antibody 14944-1-AP	Download protocol
WB protocol for GGPS1 antibody 14944-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Dev Cell Quantitative proteomic profiling identifies global protein network dynamics in murine embryonic heart development Authors - Whitney Edwards View Article
mouse	WB	FEBS J Conditional Loss of Geranylgeranyl Diphosphate Synthase Alleviates Acute Obstructive Cholestatic Liver Injury by Regulating Hepatic Bile Acid Metabolism. Authors - Wen-Jun Jia View Article
	IHC	Am J Pathol Geranylgeranyl Diphosphate Synthase Modulates Fetal Lung Branching Morphogenesis Possibly through Controlling K-Ras Prenylation. Authors - Wen-Jun Jia View Article
human	WB,IHC	J Cell Mol Med Overexpression of geranylgeranyl diphosphate synthase contributes to tumour metastasis and correlates with poor prognosis of lung adenocarcinoma. Authors - Xiaoxia Wang KD Validated View Article
mouse	WB,IF	Cells Cysteine-Rich LIM-Only Protein 4 (CRP4) Promotes Atherogenesis in the ApoE-/- Mouse Model. Authors - Natalie Längst View Article
human	WB	J Hepatocell Carcinoma miR-612 Enhances RSL3-Induced Ferroptosis of Hepatocellular Carcinoma Cells via Mevalonate Pathway Authors - Kailin Xing View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse brain using 14944-1-AP

WB analysis of human placenta using 14944-1-AP

WB analysis of human testis using 14944-1-AP

WB analysis of mouse bladder using 14944-1-AP

WB analysis of human heart using 14944-1-AP

IP experiment of mouse brain using 14944-1-AP

IHC staining of human kidney using 14944-1-AP

IHC staining of human kidney using 14944-1-AP

IHC staining of human liver using 14944-1-AP

IHC staining of human liver using 14944-1-AP

IHC staining of human testis using 14944-1-AP

IHC staining of human testis using 14944-1-AP

GGPS1 Polyclonal antibody

GGPS1 Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Quantitative proteomic profiling identifies global protein network dynamics in murine embryonic heart development

Conditional Loss of Geranylgeranyl Diphosphate Synthase Alleviates Acute Obstructive Cholestatic Liver Injury by Regulating Hepatic Bile Acid Metabolism.

Geranylgeranyl Diphosphate Synthase Modulates Fetal Lung Branching Morphogenesis Possibly through Controlling K-Ras Prenylation.

Overexpression of geranylgeranyl diphosphate synthase contributes to tumour metastasis and correlates with poor prognosis of lung adenocarcinoma.

Cysteine-Rich LIM-Only Protein 4 (CRP4) Promotes Atherogenesis in the ApoE-/- Mouse Model.

miR-612 Enhances RSL3-Induced Ferroptosis of Hepatocellular Carcinoma Cells via Mevalonate Pathway