Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of MCF-7 using 16209-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16209-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16209-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 16209-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 16209-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 16209-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 16209-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 16209-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16209-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16209-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 16209-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 16209-1-AP
IP result of anti-GGCX (IP:16209-1-AP, 4ug; Detection:16209-1-AP 1:2000) with mouse liver tissue lysate 4000ug.
IHC staining of human liver using 16209-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16209-1-AP (GGCX antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 16209-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16209-1-AP (GGCX antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human liver using 16209-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16209-1-AP (GGCX antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 16209-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16209-1-AP (GGCX antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human brain using 16209-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 16209-1-AP (GGCX antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 16209-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 16209-1-AP (GGCX antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

GGCX Polyclonal antibody

GGCX Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 16209-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

gamma glutamyl carboxylase, GC, GGCX, VKCFD1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (12)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	MCF7 cells, HEK-293 cells, HepG2 cells, L02 cells, MCF-7 cells, mouse liver tissue, PC-3 cells
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human liver tissue, human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 9 publications below
IHC	See 1 publications below
IF	See 1 publications below
IP	See 2 publications below

Product Information

16209-1-AP targets GGCX in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9185 Product name: Recombinant human GGCX protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 383-758 aa of BC013979 Sequence: LTQGYNNWTNGLYGYSWDMMVHSRSHQHVKITYRDGRTGELGYLNPGVFTQSRRWKDHADMLKQYATCLSRLLPKYNVTEPQIYFDIWVSINDRFQQRIFDPRVDIVQAAWSPFQRTSWVQPLLMDLSPWRAKLQEIKSSLDNHTEVVFIADFPGLHLENFVSEDLGNTSIQLLQGEVTVELVAEQKNQTLREGEKMQLPAGEYHKVYTTSPSPSCYMYVYVNTTELALEQDLAYLQELKEKVENGSETGPLPPELQPLLEGEVKGGPEPTPLVQTFLRRQQRLQEIERRRNTPFHERFFRFLLRKLYVFRRSFLMTCISLRNLILGRPSLEQLAQEVTYANLRPFEAVGELNPSNTDSSHSNPPESNPDPVHSEF 相同性解析による交差性が予測される生物種
Full Name	gamma-glutamyl carboxylase
Calculated molecular weight	758 aa, 88 kDa
Observed molecular weight	88 kDa
GenBank accession number	BC013979
Gene Symbol	GGCX
Gene ID (NCBI)	2677
RRID	AB_2110874
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P38435
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GGCX(Gamma-glutamyl carboxylase) is also named as GC and belongs to the vitamin K-dependent gamma-carboxylase family. This 94 kDa (including all modifications, such as the five N-linked glycosylations), is a 5-pass transmembrane protein and a key regulator of blood coagulation(PMID:20518534). It mediates the vitamin K-dependent carboxylation of glutamate residues to calcium-binding gamma-carboxyglutamate (Gla) residues with the concomitant conversion of the reduced hydroquinone form of vitamin K to vitamin K epoxide. Defects in GGCX are a cause of combined deficiency of vitamin K-dependent clotting factors type 1 (VKCFD1) and pseudoxanthoma elasticum-like disorder with multiple coagulation factor deficiency (PXEL-MCFD)(PMID:9845520;17110937).

Protocols

Product Specific Protocols
IHC protocol for GGCX antibody 16209-1-AP	Download protocol
IP protocol for GGCX antibody 16209-1-AP	Download protocol
WB protocol for GGCX antibody 16209-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	J Cell Biol GGCX and VKORC1 inhibit osteocalcin endocrine functions. Authors - Mathieu Ferron View Article
		Blood Characterization of vitamin K-dependent carboxylase mutations that cause bleeding and nonbleeding disorders. Authors - Jian-Ke Tie View Article
human	WB	Methods Enzymol Functional Study of the Vitamin K Cycle Enzymes in Live Cells. Authors - J-K Tie KO Validated View Article
human	WB	JCI Insight VKOR paralog VKORC1L1 supports vitamin K-dependent protein carboxylation in vivo. Authors - Julie Lacombe View Article
human	WB	Oncotarget Divergent effects of vitamins K1 and K2 on triple negative breast cancer cells. Authors - Sarah Beaudin View Article
human,mouse	WB,IP	Cell Rep Vitamin K-dependent carboxylation regulates Ca2+ flux and adaptation to metabolic stress in β cells Authors - Julie Lacombe View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of MCF-7 using 16209-1-AP

WB analysis of HepG2 using 16209-1-AP

WB analysis of HepG2 using 16209-1-AP

WB analysis of MCF-7 using 16209-1-AP

WB analysis of HEK-293 using 16209-1-AP

WB analysis of L02 using 16209-1-AP

WB analysis of PC-3 using 16209-1-AP

WB analysis of PC-3 using 16209-1-AP

WB analysis of mouse liver using 16209-1-AP

WB analysis of mouse liver using 16209-1-AP

WB analysis of MCF-7 using 16209-1-AP

IP experiment of mouse liver using 16209-1-AP

IHC staining of human liver using 16209-1-AP

IHC staining of human liver using 16209-1-AP

IHC staining of human liver using 16209-1-AP

IHC staining of human liver using 16209-1-AP

IHC staining of human brain using 16209-1-AP

IHC staining of human brain using 16209-1-AP

GGCX Polyclonal antibody

GGCX Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

GGCX and VKORC1 inhibit osteocalcin endocrine functions.

Characterization of vitamin K-dependent carboxylase mutations that cause bleeding and nonbleeding disorders.

Functional Study of the Vitamin K Cycle Enzymes in Live Cells.

VKOR paralog VKORC1L1 supports vitamin K-dependent protein carboxylation in vivo.

Divergent effects of vitamins K1 and K2 on triple negative breast cancer cells.

Vitamin K-dependent carboxylation regulates Ca2+ flux and adaptation to metabolic stress in β cells