Validation Data Gallery

Filter: AllWB

WB analysis of K-562 using 14198-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 14198-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 14198-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 14198-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 14198-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 14198-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 14198-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of RAW264.7 using 14198-1-AP
RAW264.7 cells were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 14198-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

GFI1 Polyclonal antibody

GFI1 Polyclonal Antibody for WB, ELISA

Cat No. 14198-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

GFI 1, GFI1, Zinc finger protein 163, Zinc finger protein Gfi 1, ZNF163

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in

K-562 cells, HL-60 cells, mouse thymus tissue, PC-3 cells, Raji cells, RAW264.7

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHC	See 1 publications below

Product Information

14198-1-AP targets GFI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5401 Product name: Recombinant human GFI1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-422 aa of BC032751 Sequence: MPRSFLVKSKKAHSYHQPRSPGPDYSLRLENVPAPSRADSTSNAGGAKAEPRDRLSPESQLTEAPDRASASPDSCEGSVCERSSEFEDFWRPPSPSASPASEKSMCPSLDEAQPFPLPFKPYSWSGLAGSDLRHLVQSYRPCGALERGAGLGLFCEPAPEPGHPAALYGPKRAAGGAGAGAPGSCSAGAGATAGPGLGLYGDFGSAAAGLYERPTAAAGLLYPERGHGLHADKGAGVKVESELLCTRLLLGGGSYKCIKCSKVFSTPHGLEVHVRRSHSGTRPFACEMCGKTFGHAVSLEQHKAVHSQERSFDCKICGKSFKRSSTLSTHLLIHSDTRPYPCQYCGKRFHQKSDMKKHTFIHTGEKPHKCQVCGKAFSQSSNLITHSRKHTGFKPFGCDLCGKGFQRKVDLRRHRETQHGLK 相同性解析による交差性が予測される生物種
Full Name	growth factor independent 1 transcription repressor
Calculated molecular weight	45 kDa
Observed molecular weight	62-66 kDa
GenBank accession number	BC032751
Gene Symbol	GFI1
Gene ID (NCBI)	2672
RRID	AB_2109976
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q99684
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GFI is a transcriptional repressor that is transiently induced during T-cell differentiation. It has critical roles in enhancing T helper-2 (Th2) cell expansion and in repressing induction of Th17 and CD103-positive inducible regulatory T (Treg) cells [PMID: 19188499]. GFI1 transcriptional regulator oncoprotein plays a crucial role in hematopoietic, inner ear, and pulmonary neuroendocrine cell development and governs cell processes as diverse as self-renewal of hematopoietic stem cells, proliferation, apoptosis, differentiation, cell fate specification, and oncogenesis (PMID: 18805794). The calculated molecular weight of GFI1 protein is about 45 kDa, but the modification of ubiquitinated GIF1 protein is about 62-66 kDa (PMID: 12721361).

Protocols

Product Specific Protocols
WB protocol for GFI1 antibody 14198-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	Nature Divergent transcriptional regulation of astrocyte reactivity across disorders. Authors - Joshua E Burda View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of K-562 using 14198-1-AP

WB analysis of mouse thymus using 14198-1-AP