GEFT-Specific Polyclonal antibody

Name: GEFT-Specific Polyclonal antibody
Brand: Proteintech
SKU: 14839-1-AP
Rating: 5.00 (1 reviews)

GEFT-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 14839-1-AP

Synonyms

ARHGEF25, GEFT, GEFT-Specific, p63RhoGEF, RhoA/RAC/CDC42 exchange factor

Validation Data Gallery

Applications:

AllWBIPIHC

WB analysis of mouse skeletal muscle using 14839-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 14839-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 14839-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 14839-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 14839-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 14839-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 14839-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 14839-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 14839-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 14839-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 14839-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 14839-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 14839-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 14839-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 14839-1-AP
IP result of anti-GEFT-Specific (IP:14839-1-AP, 4ug; Detection:14839-1-AP 1:1500) with mouse brain tissue lysate 8000ug.
IHC staining of human pancreas using 14839-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas slide using 14839-1-AP (GEFT-Specific Antibody) at dilution of 1:100.
IHC staining of human pancreas using 14839-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas slide using 14839-1-AP (GEFT-Specific Antibody) at dilution of 1:100.
IHC staining of human pancreas using 14839-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas slide using 14839-1-AP (GEFT-Specific Antibody) at dilution of 1:100.
IHC staining of human pancreas using 14839-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas slide using 14839-1-AP (GEFT-Specific Antibody) at dilution of 1:100.
IHC staining of human skeletal muscle using 14839-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 14839-1-AP (GEFT-Specific Antibody) at dilution of 1:100.
IHC staining of human skeletal muscle using 14839-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 14839-1-AP (GEFT-Specific Antibody) at dilution of 1:100.

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Cat No. 14839-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse skeletal muscle tissue, HEK-293 cells, mouse heart tissue, mouse liver tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human pancreas tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 1 publications below
WB	See 13 publications below
IHC	See 3 publications below
IF	See 3 publications below

Product Information

14839-1-AP targets GEFT-Specific in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	RhoA/RAC/CDC42 exchange factor
Calculated molecular weight	64 kDa
Observed molecular weight	64 kDa
GenBank accession number	NM_182947
Gene symbol	GEFT
Gene ID (NCBI)	115557
RRID	AB_2089323
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GEFT, also named as p63RhoGEF, may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. GETF links specifically G alpha q/11-coupled receptors to RHOA activation. GEFT may be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. It is involved in skeletal myogenesis. GEFT is expressed as three isoforms (64, 53 and 68 kDa).

Protocols

Product Specific Protocols
WB protocol for GEFT-Specific antibody 14839-1-AP	Download protocol
IHC protocol for GEFT-Specific antibody 14839-1-AP	Download protocol
IP protocol for GEFT-Specific antibody 14839-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Genes Dev TNF-stimulated MAP kinase activation mediated by a Rho family GTPase signaling pathway. Authors - Kant Shashi S View Article
human	WB	Circ Res p63RhoGEF couples Gα(q/11)-mediated signaling to Ca2+ sensitization of vascular smooth muscle contractility. Authors - Momotani Ko K View Article
mouse	WB	Sci Signal RSK2 contributes to myogenic vasoconstriction of resistance arteries by activating smooth muscle myosin and the Na+/H+ exchanger. Authors - Mykhaylo V Artamonov View Article
rat	WB,IHC	FASEB J p63RhoGEF--a key mediator of angiotensin II-dependent signaling and processes in vascular smooth muscle cells. Authors - Wuertz Christina M CM View Article
mouse	WB,IHC,IF	Mol Cell Biol Modulation of muscle regeneration, myogenesis, and adipogenesis by the Rho family guanine nucleotide exchange factor GEFT. Authors - Bryan Brad A BA View Article
human	WB	J Hypertens Increased level of p63RhoGEF and RhoA/Rho kinase activity in hypertensive patients. Authors - Lorenzo A Calò View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Joelle (Verified Customer) (09-10-2019)

Overnight at 4 degrees

Applications: Western Blot,
Primary Antibody Dilution: 1/1000
Cell Tissue Type: HHL16

GEFT-Specific Antibody Western Blot, validation (1/1000 dilution) in HHL16 (Cat no:14839-1-AP)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GEFT-Specific Polyclonal antibody

GEFT-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis of mouse skeletal muscle using 14839-1-AP

WB analysis of mouse skeletal muscle using 14839-1-AP

WB analysis of HEK-293 using 14839-1-AP

WB analysis of HEK-293 using 14839-1-AP

WB analysis of mouse skeletal muscle using 14839-1-AP

WB analysis of mouse skeletal muscle using 14839-1-AP

WB analysis of mouse skeletal muscle using 14839-1-AP

WB analysis of mouse skeletal muscle using 14839-1-AP

WB analysis of mouse heart using 14839-1-AP

WB analysis of mouse heart using 14839-1-AP

WB analysis of mouse heart using 14839-1-AP

WB analysis of mouse heart using 14839-1-AP

WB analysis of mouse liver using 14839-1-AP

WB analysis of mouse liver using 14839-1-AP

IP experiment of mouse brain using 14839-1-AP

IHC staining of human pancreas using 14839-1-AP

IHC staining of human pancreas using 14839-1-AP

IHC staining of human pancreas using 14839-1-AP

IHC staining of human pancreas using 14839-1-AP

IHC staining of human skeletal muscle using 14839-1-AP

IHC staining of human skeletal muscle using 14839-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

TNF-stimulated MAP kinase activation mediated by a Rho family GTPase signaling pathway.

p63RhoGEF couples Gα(q/11)-mediated signaling to Ca2+ sensitization of vascular smooth muscle contractility.

RSK2 contributes to myogenic vasoconstriction of resistance arteries by activating smooth muscle myosin and the Na+/H+ exchanger.

p63RhoGEF--a key mediator of angiotensin II-dependent signaling and processes in vascular smooth muscle cells.

Modulation of muscle regeneration, myogenesis, and adipogenesis by the Rho family guanine nucleotide exchange factor GEFT.

Increased level of p63RhoGEF and RhoA/Rho kinase activity in hypertensive patients.

Reviews