Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 11854-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 11854-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 11854-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis using 11854-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human lung using 11854-1-AP
human lung tissue were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IHC staining of human spleen using 11854-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11854-1-AP (GBP2 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human spleen using 11854-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11854-1-AP (GBP2 antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human tonsillitis using 11854-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11854-1-AP (GBP2 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human tonsillitis using 11854-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11854-1-AP (GBP2 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 11854-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GBP2 antibody (11854-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

GBP2 Polyclonal antibody

GBP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11854-1-AP

Publications(60)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

EC:3.6.5.-, GBP 2, GBP-2, GTP-binding protein 2, Guanine nucleotide-binding protein 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 647

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (60)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse spleen tissue, human placenta tissue, A375 cells, K-562 cells, human lung tissue, rat spleen tissue
Positive IHC detected in	human spleen tissue, human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 42 publications below
IHC	See 12 publications below
IF	See 25 publications below
IP	See 1 publications below
CoIP	See 2 publications below

Product Information

11854-1-AP targets GBP2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, toxoplasma
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2425 Product name: Recombinant human GBP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 131-481 aa of BC022272 Sequence: MGTINQQAMDQLHYVTELTDRIKANSSPGNNSVDDSADFVSFFPAFVWTLRDFTLELEVDGEPITADDYLELSLKLRKGTDKKSKSFNDPRLCIRKFFPKRKCFVFDWPAPKKYLAHLEQLKEEELNPDFIEQVAEFCSYILSHSNVKTLSGGIPVNGPRLESLVLTYVNAISSGDLPCMENAVLALAQIENSAAVEKAIAHYEQQMGQKVQLPTETLQELLDLHRDSEREAIEVFMKNSFKDVDQMFQRKLGAQLEARRDDFCKQNSKASSDCCMALLQDIFGPLEEDVKQGTFSKPGGYRLFTQKLQELKNKYYQVPRKGIQAKEVLKKYLESKEDVADALLQTDQSLS 相同性解析による交差性が予測される生物種
Full Name	guanylate binding protein 2, interferon-inducible
Calculated molecular weight	591 aa, 67 kDa
Observed molecular weight	67 kDa
GenBank accession number	BC022272
Gene Symbol	GBP2
Gene ID (NCBI)	2634
RRID	AB_2109336
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P32456
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GBP2 is a member of the GTPase family and is crucial to host immunity against pathogens. GBP2 is also used as a marker of interferon (IFN) responsiveness because it is one of the most highly expressed genes after IFN-γ stimulation. Increased GBP2 expression is also associated with a better prognosis in breast cancer and may have a role in T-cell defense against breast cancer. Interestingly, GBP2 expression in NIH/3T3 fibroblasts inhibited Rac activation and matrix metalloproteinase-9 expression, suggesting a possible role for GBP2 in regulating cancer metastasis. The molecular weight of GBP2 is 67 kDa. (PMID: 29072687, 35383115)

Protocols

Product Specific Protocols
IF protocol for GBP2 antibody 11854-1-AP	Download protocol
IHC protocol for GBP2 antibody 11854-1-AP	Download protocol
WB protocol for GBP2 antibody 11854-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Nature Ubiquitination and degradation of GBPs by a Shigella effector to suppress host defence. Authors - Peng Li View Article
mouse	WB,IHC,IF	Nature Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases. Authors - Etienne Meunier View Article
mouse	WB	Cell IRF8 Regulates Transcription of Naips for NLRC4 Inflammasome Activation. Authors - Rajendra Karki View Article
human	WB	Cell IRGB10 Liberates Bacterial Ligands for Sensing by the AIM2 and Caspase-11-NLRP3 Inflammasomes. Authors - Si Ming Man View Article
mouse	WB	Nat Immunol The transcription factor IRF1 and guanylate-binding proteins target activation of the AIM2 inflammasome by Francisella infection. Authors - Si Ming Man View Article
mouse	IF	Nat Neurosci A phenotypic screening platform for identifying chemical modulators of astrocyte reactivity Authors - Benjamin L L Clayton View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 11854-1-AP

WB analysis of K-562 using 11854-1-AP

WB analysis of human placenta using 11854-1-AP

WB analysis using 11854-1-AP

WB analysis of human lung using 11854-1-AP

IHC staining of human spleen using 11854-1-AP

IHC staining of human spleen using 11854-1-AP

IHC staining of human tonsillitis using 11854-1-AP

IHC staining of human tonsillitis using 11854-1-AP

IF Staining of HeLa using 11854-1-AP

GBP2 Polyclonal antibody

GBP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Ubiquitination and degradation of GBPs by a Shigella effector to suppress host defence.

Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases.

IRF8 Regulates Transcription of Naips for NLRC4 Inflammasome Activation.

IRGB10 Liberates Bacterial Ligands for Sensing by the AIM2 and Caspase-11-NLRP3 Inflammasomes.

The transcription factor IRF1 and guanylate-binding proteins target activation of the AIM2 inflammasome by Francisella infection.

A phenotypic screening platform for identifying chemical modulators of astrocyte reactivity