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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GATC Monoclonal antibody

GATC Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F9C8

Cat no : 68405-1-Ig

Synonyms

15E1.2, FLJ37000, GATC, GatC like protein, Protein 15E1.2

Validation Data Gallery

Applications:
  • WB analysis of A549 using 68405-1-Ig

    WB analysis of A549 using 68405-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 68405-1-Ig (GATC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68405-1-Ig

    WB analysis using 68405-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68405-1-Ig (GATC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inLNCaP cells, A549 cells, HEK-293 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Sample-dependent, check data in validation data gallery

Product Information

68405-1-Ig targets GATC in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GATC fusion protein Ag19857 相同性解析による交差性が予測される生物種
Full Name glutamyl-tRNA(Gln) amidotransferase, subunit C homolog (bacterial)
Calculated molecular weight 136 aa, 15 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC107145
Gene symbol GATC
Gene ID (NCBI) 283459
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GATC encodes a subunit of the glutamyl-tRNA(Gln) amidotransferase protein complex (GatCAB), which plays a role in aminoacylation of mitochondrial glutaminyl mt-tRNA. The complex plays an role in mitochondrial translation and protein synthesis (PubMed: 30283131).

Protocols

Product Specific Protocols
WB protocol for GATC antibody 68405-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols