GATC Polyclonal antibody
GATC Polyclonal Antibody for IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 23183-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | human liver tissue, human appendicitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
23183-1-AP targets GATC in IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | GATC fusion protein Ag19713 相同性解析による交差性が予測される生物種 |
Full Name | glutamyl-tRNA(Gln) amidotransferase, subunit C homolog (bacterial) |
Calculated molecular weight | 136 aa, 15 kDa |
Observed molecular weight | 15 kDa |
GenBank accession number | BC107145 |
Gene symbol | GATC |
Gene ID (NCBI) | 283459 |
RRID | AB_2879226 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen Affinity purified |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GATC encodes a subunit of the glutamyl-tRNA(Gln) amidotransferase protein complex (GatCAB), which plays a role in aminoacylation of mitochondrial glutaminyl mt-tRNA. The complex plays an role in mitochondrial translation and protein synthesis (PubMed: 30283131).
Protocols
Product Specific Protocols | |
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IHC protocol for GATC antibody 23183-1-AP | Download protocol |
IP protocol for GATC antibody 23183-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |