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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GALE Monoclonal antibody

GALE Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig, Rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3F2C2

Cat no : 67772-1-Ig

Synonyms

FLJ95174, FLJ97302, Galactowaldenase, GALE, SDR1E1, UDP galactose 4 epimerase, UDP glucose 4 epimerase

Validation Data Gallery

Applications:
  • WB analysis using 67772-1-Ig

    WB analysis using 67772-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67772-1-Ig (GALE antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inU2OS cells, A375 cells, HEK-293T cells, HeLa cells, K-562 cells, 4T1 cells, rat liver tissue, pig liver tissue, rabbit liver tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

67772-1-Ig targets GALE in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

Tested Reactivity Human, Mouse, Rat, Pig, Rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GALE fusion protein Ag6571 相同性解析による交差性が予測される生物種
Full Name UDP-galactose-4-epimerase
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC050685
Gene symbol GALE
Gene ID (NCBI) 2582
RRIDAB_2918537
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GALE(Galactowaldenase) is also named as UDP-glucose 4-epimerase, UDP-galactose 4-epimerase, and belongs to the sugar epimerase family. UDP-galactose 4-epimerase catalyzes the interconversion of UDP-glucose and UDP-galactose during normal galactose metabolism. In humans, deficiencies in this enzyme lead to the complex disorder referred to as epimerase-deficiency galactosemia(PMID:10801319).It can exsit as a homodimer(PMID:8931134).

Protocols

Product Specific Protocols
WB protocol for GALE antibody 67772-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols