GABRA1 Polyclonal antibody

GABRA1 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 12410-1-AP

Synonyms

ECA4, EJM, GABA A receptor alpha 1, GABAA receptor alpha 1, GABRA1



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inrat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

12410-1-AP targets GABRA1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GABRA1 fusion protein Ag3117 相同性解析による交差性が予測される生物種
Full Name gamma-aminobutyric acid (GABA) A receptor, alpha 1
Calculated molecular weight 456 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC030696
Gene symbol GABRA1
Gene ID (NCBI) 2554
RRIDAB_2108692
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GABRA1 antibody 12410-1-APDownload protocol
IHC protocol for GABRA1 antibody 12410-1-APDownload protocol
IP protocol for GABRA1 antibody 12410-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Biotechnol

Magnify is a universal molecular anchoring strategy for expansion microscopy

Authors - Aleksandra Klimas
mouseWB

Nat Metab

Gut microbiome-derived ammonia modulates stress vulnerability in the host

Authors - Pei Wang
human

Neurol Neuroimmunol Neuroinflamm

MRI Characteristics of Autoimmune Encephalitis With Autoantibodies to GABAA Receptor: A Case Series.

Authors - Bo Deng
mouseWB

Mol Ther Nucleic Acids

Modulation of miR-181 influences dopaminergic neuronal degeneration in a mouse model of Parkinson's disease.

Authors - Colleen S Stein
mouseWB

Int J Mol Sci

The Effect of TGF-β1 Reduced Functionality on the Expression of Selected Synaptic Proteins and Electrophysiological Parameters: Implications of Changes Observed in Acute Hepatic Encephalopathy.

Authors - Mariusz Popek
mouseWB

Cell Death Dis

Transgenic overexpression of furin increases epileptic susceptibility.

Authors - Yi Yang