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  • KD/KO Validated

GABPB1 Polyclonal antibody

GABPB1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12597-1-AP

Synonyms

BABPB2, E4TF1, E4TF1 47, E4TF1 53, E4TF1B, GABP subunit beta 1, GABP subunit beta 2, GABPB, GABPB 1, GABPB 2, GABPB1, GABPB2, NRF2B1, NRF2B2, Nuclear respiratory factor 2, Transcription factor E4TF1 47, Transcription factor E4TF1 53



Tested Applications

Positive WB detected inA375 cells, MCF-7 cells, mouse lung tissue, HeLa cells, HepG2 cells, mouse testis tissue, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GABPB1 Ready-To-Use IHC Kit. GABPB1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

12597-1-AP targets GABPB1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GABPB1 fusion protein Ag3208 相同性解析による交差性が予測される生物種
Full Name GA binding protein transcription factor, beta subunit 1
Calculated molecular weight 347 aa, 38 kDa
Observed molecular weight 36-45 kDa, 47-53 kDa
GenBank accession numberBC016910
Gene symbol GABPB1
Gene ID (NCBI) 2553
RRIDAB_10951115
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GABPB1, also named as E4TF1B, GABPB, GABPB2, E4TF1-47 and E4TF1-53, is a transcription factor capable of interacting with purine rich repeats (GA repeats). It is necessary for the expression of the Adenovirus E4 gene. GABPB1 has 4 isoforms. The MW-of Beta 1 is 53kd and Beta-2 is 47kd(PMID:9525935). This antibody can recognize all the isoforms.

Protocols

Product Specific Protocols
WB protocol for GABPB1 antibody 12597-1-APDownload protocol
IHC protocol for GABPB1 antibody 12597-1-APDownload protocol
IF protocol for GABPB1 antibody 12597-1-APDownload protocol
IP protocol for GABPB1 antibody 12597-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Cell

Disruption of the β1L Isoform of GABP Reverses Glioblastoma Replicative Immortality in a TERT Promoter Mutation-Dependent Manner.

Authors - Andrew Mancini
  • KD Validated
humanWB

Proc Natl Acad Sci U S A

Cancer-specific loss of TERT activation sensitizes glioblastoma to DNA damage.

Authors - Alexandra M Amen
human, mouseWB,IHC,IF

Cell Rep

The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.

Authors - Wu Hongtan H
  • KD Validated
humanWB

Sci Rep

LncRNA GABPB1-AS1 and GABPB1 regulate oxidative stress during erastin-induced ferroptosis in HepG2 hepatocellular carcinoma cells.

Authors - Wenchuan Qi
  • KD Validated
humanWB

Biology (Basel)

TERT Promoter Revertant Mutation Inhibits Melanoma Growth through Intrinsic Apoptosis.

Authors - Yanbing Wang
mouseWB

Brain Res Bull

ZFP36 protects against oxygen-glucose deprivation/reoxygenation-induced mitochondrial fragmentation and neuronal apoptosis through inhibiting NOX4-DRP1 pathway.

Authors - Hengjiang Guo