Anti-Mouse Foxp3 (3G3)

Foxp3 Monoclonal Antibody for FC, IHC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Mouse and More (1)

Applications

IHC, IF, FC

Conjugate

Unconjugated

CloneNo.

3G3

Cat no : 65089-1-Ig

Synonyms

forkhead box P3, Foxp3, JM2, scurfin, sf



Tested Applications

Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inmouse splenocytes
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

65089-1-Ig targets Foxp3 in IHC, IF, FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Cited Reactivity mouse
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name forkhead box P3
GenBank accession numberBC132333
Gene symbol Foxp3
Gene ID (NCBI) 20371
RRIDAB_2918392
Conjugate Unconjugated
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide, pH 7.3.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

Foxp3 (forkhead box P3), also named as IPEX, JM2 and scurfin, is a member of the forkhead transcription factor family and is essential for normal immune homeostasis (PMID: 11138001; 20429413). Foxp3 is specifically expressed in CD4+ CD25+ regulatory T cells and is crucial for their development and suppressive activity (PMID: 12612578; 12612581). In scurfy mouse, disruption of Foxp3 results in the fatal lymphoproliferative disorder attributed to the lack of an immune-suppressive component in the immune system (PMID: 25683611; 11138001). Similar to mice, genetic defects in human Foxp3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome (PMID: 11137993).

Protocols

Product Specific Protocols
IHC protocol for Foxp3 antibody 65089-1-IgDownload protocol
FC protocol for Foxp3 antibody 65089-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Thorac Cancer

Adiponectin-expressing Treg cells in experimental thymic tumor model

Authors - Yuki Hanamatsu
mouseIF

Adv Sci (Weinh)

Targeting L-Selectin Lymphocytes to Deliver Immunosuppressive Drug in Lymph Nodes for Durable Multiple Sclerosis Treatment

Authors - Yipeng Zhao
mouseIF

Thorac Cancer

Adiponectin-expressing Treg-containing T cell fraction inhibits tumor growth in orthotopically implanted triple-negative breast cancer

Authors - Wakana Chikaishi