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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Fhl1 Polyclonal antibody

Fhl1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 51015-1-AP

Synonyms

Fhl1, four and a half LIM domains 1, KyoT

Validation Data Gallery

Applications:
  • WB analysis of human heart using 51015-1-AP

    WB analysis of human heart using 51015-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 51015-1-AP (Fhl1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 51015-1-AP

    IHC staining of human ovary tumor using 51015-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 51015-1-AP (Fhl1 antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inhuman heart tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

51015-1-AP targets Fhl1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name four and a half LIM domains 1
Calculated molecular weight 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC031120
Gene symbol Fhl1
Gene ID (NCBI) 14199
RRIDAB_615005
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Fhl1 antibody 51015-1-APDownload protocol
IHC protocol for Fhl1 antibody 51015-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols