Validation Data Gallery

Filter: AllWBIHC

WB analysis using 13865-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 13865-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 13865-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 13865-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13865-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 13865-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse eye using 13865-1-AP
mouse eye tissue were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HUVEC using 13865-1-AP
HUVEC cells were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human gliomas using 13865-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13865-1-AP (Frizzled 9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 13865-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13865-1-AP (Frizzled 9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Frizzled 9 Polyclonal antibody

Frizzled 9 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13865-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CD349, Frizzled 9, Fz 9, FZD3, FZD9, FzE6, hFz9

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, mouse skeletal muscle tissue, mouse testis tissue, mouse brain tissue, mouse eye tissue, HUVEC cells
Positive IHC detected in	human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below

Product Information

13865-1-AP targets Frizzled 9 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4847 Product name: Recombinant human FZD9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 23-247 aa of BC026333 Sequence: LEIGRFDPERGRGAAPCQAVEIPMCRGIGYNLTRMPNLLGHTSQGEAAAELAEFAPLVQYGCHSHLRFFLCSLYAPMCTDQVSTPIPACRPMCEQARLRCAPIMEQFNFGWPDSLDCARLPTRNDPHALCMEAPENATAGPAEPHKGLGMLPVAPRPARPPGDLGPGAGGSGTCENREKFQYVEKSRSCAPRCGPGVEVFWSRRDKDFALVWMAVWSALCFFSTA 相同性解析による交差性が予測される生物種
Full Name	frizzled homolog 9 (Drosophila)
Calculated molecular weight	591 aa, 64 kDa
Observed molecular weight	64 kDa
GenBank accession number	BC026333
Gene Symbol	Frizzled 9
Gene ID (NCBI)	8326
RRID	AB_2107358
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O00144
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for Frizzled 9 antibody 13865-1-AP	Download protocol
WB protocol for Frizzled 9 antibody 13865-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Small Zinc-Cobalt Bimetallic Organic Frameworks with Antioxidative and Osteogenic Activities for Periodontitis Treatment Authors - Hao Tang View Article
human	WB	Front Oncol YTHDF1 Regulates Tumorigenicity and Cancer Stem Cell-Like Activity in Human Colorectal Carcinoma. Authors - Yang Bai View Article
human	WB	Theranostics Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3β Complex in Hypophosphatasia. Authors - Wenjia Liu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 13865-1-AP

WB analysis of mouse testis using 13865-1-AP

WB analysis of mouse testis using 13865-1-AP

WB analysis of mouse skeletal muscle using 13865-1-AP

WB analysis of HeLa using 13865-1-AP

WB analysis of mouse brain using 13865-1-AP

WB analysis of mouse eye using 13865-1-AP

WB analysis of HUVEC using 13865-1-AP

IHC staining of human gliomas using 13865-1-AP