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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FUCA1 Monoclonal antibody

FUCA1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G5C5

Cat no : 67251-1-Ig

Synonyms

Alpha L fucosidase 1, Alpha L fucosidase I, FUCA, FUCA1, fucosidase, alpha L 1, tissue, Tissue alpha L fucosidase

Validation Data Gallery

Applications:
  • WB analysis of HepG2 using 67251-1-Ig

    WB analysis of HepG2 using 67251-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67251-1-Ig (FUCA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 67251-1-Ig

    WB analysis of K-562 using 67251-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67251-1-Ig (FUCA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 67251-1-Ig

    WB analysis of NIH/3T3 using 67251-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67251-1-Ig (FUCA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 67251-1-Ig

    WB analysis of HEK-293 using 67251-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67251-1-Ig (FUCA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67251-1-Ig

    IHC staining of human liver cancer using 67251-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67251-1-Ig (FUCA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FUCA1 antibody included
  • IHC staining of human liver cancer using 67251-1-Ig

    IHC staining of human liver cancer using 67251-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67251-1-Ig (FUCA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FUCA1 antibody included
  • IF Staining of human liver cancer using 67251-1-Ig

    IF Staining of human liver cancer using 67251-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FUCA1 antibody (67251-1-Ig, Clone: 1G5C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human liver cancer using 67251-1-Ig

    IF Staining of human liver cancer using 67251-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FUCA1 antibody (67251-1-Ig, Clone: 1G5C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, K-562 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue
Planning an IHC experiment? We recommend our IHCeasy FUCA1 Ready-To-Use IHC Kit. FUCA1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67251-1-Ig targets FUCA1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FUCA1 fusion protein Ag9638 相同性解析による交差性が予測される生物種
Full Name fucosidase, alpha-L- 1, tissue
Calculated molecular weight 461 aa, 53 kDa
Observed molecular weight 50-56 kDa
GenBank accession numberBC017338
Gene symbol FUCA1
Gene ID (NCBI) 2517
RRIDAB_2882528
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

FUCA1(a-l-Fucosidase) is a lysosomal glycosidase which is ubiquitous in eukaryotic cells. This enzyme is usually found as a soluble component of the lysosome and functions as an acid hydrolase in the degradation of numerous and diverse fucoglycoconjugates.The mammalian a-l-fucosidases are relatively large, multisubunit glycoproteins which exist in multiple molecular isoforms.This enzyme may have a role in sperm-egg interactions in the reproductive tract of the female rat. There is a precursor 54 kDa form of rat sperm fucosidase which is processed to a mature 52 kDa form of the enzyme(PMID:8836125). This protein from rat epididymis is a tetramer of 210-220 kDa, made up by two pairs of subunits of 47 and 60 kDa, and the one from human seminal plasma exists in different oligomeric forms, that is, as a dimer, as a tetramer, and as a hexamer made up of a single subunit of 56-57 kDa(PMID:16736526).

Protocols

Product Specific Protocols
WB protocol for FUCA1 antibody 67251-1-IgDownload protocol
IHC protocol for FUCA1 antibody 67251-1-IgDownload protocol
IF protocol for FUCA1 antibody 67251-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols