Validation Data Gallery

Filter: AllWBIP

WB analysis of HeLa using 13147-1-AP
WB result of FOXM1 antibody (13147-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXM1 transfected HeLa cells.
WB analysis using 13147-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13147-1-AP (FOXM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13147-1-AP
Non-treated HeLa and thymidine or nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 13147-1-AP (FOXM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control.
WB analysis using 13147-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13147-1-AP (FOXM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 13147-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13147-1-AP (FOXM1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 13147-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13147-1-AP (FOXM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 13147-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 13147-1-AP (FOXM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 13147-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 13147-1-AP (FOXM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 13147-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13147-1-AP (FOXM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 13147-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 13147-1-AP (FOXM1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of L02 using 13147-1-AP
IP result of anti-FOXM1 (IP:13147-1-AP, 4ug; Detection:13147-1-AP 1:1000) with L02 cells lysate 1040 ug.

Featured Product
KD/KO Validated

FOXM1 Polyclonal antibody

FOXM1 Polyclonal Antibody for WB, IP, ELISA

Cat No. 13147-1-AP

Publications(134)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, CoIP, chIP, RIP, ELISA

Synonyms

FKHL16, Forkhead-related protein FKHL16, HFH11, HFH-11, HNF-3/fork-head homolog 11

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (134)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HT-29 cells, HEK-293 cells, HeLa cells, human testis tissue, mouse colon tissue, mouse spleen tissue, mouse testis tissue, mouse thymus tissue, MCF-7 cells, L02 cells, A431 cells
Positive IP detected in	L02 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 24 publications below
WB	See 109 publications below
IP	See 3 publications below
CoIP	See 1 publications below
ChIP	See 13 publications below
RIP	See 1 publications below

Product Information

13147-1-AP targets FOXM1 in WB, IP, CoIP, chIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3729 Product name: Recombinant human FOXM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 447-748 aa of BC006192 Sequence: EEIQPGEEMPHLARPIKVESPPLEEWPSPAPSFKEESSHSWEDSSQSPTPRPKKSYSGLRSPTRCVSEMLVIQHRERRERSRSRRKQHLLPPCVDEPELLFSEGPSTSRWAAELPFPADSSDPASQLSYSQEVGGPFKTPIKETLPISSTPSKSVLPRTPESWRLTPPAKVGGLDFSPVQTPQGASDPLPDPLGLMDLSTTPLQSAPPLESPQRLLSSEPLDLISVPFGNSSPSDIDVPKPGSPEPQVSGLAANRSLTEGLVLDTMNDSLSKILLDISFPGLDEDPLGPDNINWSQFIPELQ 相同性解析による交差性が予測される生物種
Full Name	forkhead box M 1
Calculated molecular weight	83 kDa
Observed molecular weight	84-110 kDa
GenBank accession number	BC006192
Gene Symbol	FOXM1
Gene ID (NCBI)	2305
RRID	AB_2106213
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q08050
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Forkhead box M1 (FOXM1), also named as HNF-3, HFH-11 or Trident, belongs to a superfamily of Fox transcription factors. FOXM1 is strongly associated with cell proliferation, it plays a important role in cell cycle progression by regulating both G1/S and G2/M phases, it is also involved in DNA damage repair, angiogenesis, apoptosis and tissue regeneration. Recently, crucial roles of this transcription factor were found in the development and progression of many cancers including colorectal, lung, prostate, liver and breast cancer. FOXM1 levels are significantly higher and associated with tumor grade in most human tumors, rendering it a potential target of cancer diagnosis and therapies. Catalog#13147-1-AP is a rabbit polyclonal antibody raised against C-terminal of human FOXM1. And this antibody can recognize three kinds of FOXM1, FOXM1-pp(~95-110kDa), FOXM1-B(isoform2,~83kDa) and FOXM1A(isoform4, ~90kDa). Sometimes a molecular weight of about 65 kDa can also be observed, which may be a degradation form of FOXM1 (PMID: 26547933).

Protocols

Product Specific Protocols
IP protocol for FOXM1 antibody 13147-1-AP	Download protocol
WB protocol for FOXM1 antibody 13147-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Signal Transduct Target Ther Abrogation of aberrant glycolytic interactions eliminates senescent cells and alleviates aging-related dysfunctions. Authors - Takumi Mikawa KD Validated View Article
mouse	WB	Cancer Discov Interferon-a potentiates anti-PD-1 efficacy by remodeling glucose metabolism in the hepatocellular carcinoma microenvironment. Authors - Bo Hu KD Validated View Article
mouse	WB	Nat Aging In vivo cyclic induction of the FOXM1 transcription factor delays natural and progeroid aging phenotypes and extends healthspan Authors - Rui Ribeiro View Article
		Nat Aging The secreted metabolite sensor CtBP2 links metabolism to healthy lifespan Authors - Motohiro Sekiya View Article
human	WB	Nat Commun LncBRM initiates YAP1 signalling activation to drive self-renewal of liver cancer stem cells. Authors - Pingping Zhu View Article
mouse	WB	Nat Commun Vagus-macrophage-hepatocyte link promotes post-injury liver regeneration and whole-body survival through hepatic FoxM1 activation. Authors - Tomohito Izumi View Article

Validation Data Gallery

WB analysis of HeLa using 13147-1-AP

WB analysis using 13147-1-AP

WB analysis of HeLa using 13147-1-AP

WB analysis using 13147-1-AP

WB analysis using 13147-1-AP

WB analysis of mouse thymus using 13147-1-AP

WB analysis of mouse colon using 13147-1-AP

WB analysis of mouse testis using 13147-1-AP

WB analysis of mouse spleen using 13147-1-AP

WB analysis of human testis using 13147-1-AP

IP experiment of L02 using 13147-1-AP

FOXM1 Polyclonal antibody

FOXM1 Polyclonal Antibody for WB, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Abrogation of aberrant glycolytic interactions eliminates senescent cells and alleviates aging-related dysfunctions.

Interferon-a potentiates anti-PD-1 efficacy by remodeling glucose metabolism in the hepatocellular carcinoma microenvironment.

In vivo cyclic induction of the FOXM1 transcription factor delays natural and progeroid aging phenotypes and extends healthspan

The secreted metabolite sensor CtBP2 links metabolism to healthy lifespan

LncBRM initiates YAP1 signalling activation to drive self-renewal of liver cancer stem cells.

Vagus-macrophage-hepatocyte link promotes post-injury liver regeneration and whole-body survival through hepatic FoxM1 activation.

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