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- KD/KO Validated
CoraLite® Plus 488-conjugated FOX2/RBM9 Monoclonal antibody
FOX2/RBM9 Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2D7G5
Cat no : CL488-66976
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive IF detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy FOX2/RBM9 Ready-To-Use IHC Kit. FOX2/RBM9 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66976-1-Ig
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-66976 targets FOX2/RBM9 in IF applications and shows reactivity with Human, Mouse samples.
| Tested Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | FOX2/RBM9 fusion protein Ag28336 相同性解析による交差性が予測される生物種 |
| Full Name | RNA binding motif protein 9 |
| Calculated molecular weight | 390 aa, 41 kDa |
| Observed molecular weight | 48 kDa, 50 and 60 kDa |
| GenBank accession number | BC013115 |
| Gene symbol | RBM9 |
| Gene ID (NCBI) | 23543 |
| RRID | AB_2919399 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
Background Information
RBM9, also named as RBFOX2 and FOX2, is a RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. There are several isoforms of RBM9 produced by variable use of multiple alternatively spliced internal exons and alternative splice sites. RBM9 may play an important role in tissue specific alternative splicing regulation.(PMID: 19688295, 21623373, 16537540)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 FOX2/RBM9 antibody CL488-66976 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
