FNDC5 Polyclonal antibody

FNDC5 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 23995-1-AP

Synonyms

FNDC5, FRCP2, Irisin



Tested Applications

Positive WB detected inmouse liver tissue, mouse stomach tissue, mouse skeletal muscle tissue, rat liver tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

23995-1-AP targets FNDC5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FNDC5 fusion protein Ag21195 相同性解析による交差性が予測される生物種
Full Name fibronectin type III domain containing 5
Calculated molecular weight 212 aa, 24 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC062297
Gene symbol FNDC5
Gene ID (NCBI) 252995
RRIDAB_2879394
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

fibronectin type III domain containing 5(FNDC5)encodes 23kDa protein, which is a membrane protein that is cleaved and secreted as a newly identified hormone, irisin. The exercise induces muscle FNDC5 expression. Exercise-induced FNDC5 gene expression in muscles was accompanied by a parallel increase in the concentration of circulating irisin, which in turn activates adipocyte thermogenic programs, leading to mitochondrial heat production and energy expenditure.

Protocols

Product Specific Protocols
WB protocol for FNDC5 antibody 23995-1-APDownload protocol
IHC protocol for FNDC5 antibody 23995-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Clin Transl Med

CRISPRa-based activation of Fgf21 and Fndc5 ameliorates obesity by promoting adipocytes browning

Authors - Hongtao Zhu
mouseWB

Neuropathol Appl Neurobiol

Irisin treatment lowers levels of phosphorylated tau in the hippocampus of pre-symptomatic female but not male htau mice.

Authors - Katie A Bretland
mouseWB

Br J Pharmacol

PMID: 31339170

Authors - Shengnan Shen
mouseWB

Aging (Albany NY)

Vitellogenin 2 promotes muscle development and stimulates the browning of white fat

Authors - Yilei Li

J Am Heart Assoc

Irisin Lowers Blood Pressure by Improvement of Endothelial Dysfunction via AMPK-Akt-eNOS-NO Pathway in the Spontaneously Hypertensive Rat.

Authors - Jinjuan Fu
mouseIHC

Biochim Biophys Acta Mol Basis Dis

Irisin is induced in renal ischemia-reperfusion to protect against tubular cell injury via suppressing p53.

Authors - Yuxue Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Mohammad (Verified Customer) (12-02-2019)

1:600 in mice hippocampusworks well with no non-specific band

  • Applications: Western Blot,