Featured Product
KD/KO Validated

FKBP5 Polyclonal antibody

Name: FKBP5 Polyclonal antibody
Brand: Proteintech
SKU: 14155-1-AP

FKBP5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14155-1-AP

Synonyms

51 kDa FK506 binding protein, 51 kDa FKBP, AIG6, Androgen regulated protein 6, FF1 antigen, FK506 binding protein 5, FKBP 5, FKBP 51, FKBP5, FKBP51, FKBP54, HSP90 binding immunophilin, P54, PPIase, PPIase FKBP5, Ptg 10, Rotamase

Validation Data Gallery

Applications:

AllWBIPIHC

WB analysis of K-562 using 14155-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 14155-1-AP (FKBP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 14155-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14155-1-AP (FKBP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 14155-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14155-1-AP (FKBP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 14155-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14155-1-AP (FKBP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 14155-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14155-1-AP (FKBP5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 14155-1-AP
IP result of anti-FKBP5 (IP:14155-1-AP, 3ug; Detection:14155-1-AP 1:1000) with K-562 cells lysate 1500ug.
IHC staining of human colon cancer using 14155-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 14155-1-AP (FKBP5 antibody) at dilution of 1:50 (under 10x lens).
Ready-to-use IHC kits available with FKBP5 antibody included
IHC staining of human colon cancer using 14155-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 14155-1-AP (FKBP5 antibody) at dilution of 1:50 (under 40x lens).
Ready-to-use IHC kits available with FKBP5 antibody included

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Cat No. 14155-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	K-562 cells, HEK-293 cells, mouse thymus tissue
Positive IP detected in	K-562 cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Planning an IHC experiment? We recommend our IHCeasy FKBP5 Ready-To-Use IHC Kit. FKBP5 primary antibody included.

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 1 publications below
WB	See 10 publications below
IHC	See 4 publications below
IF	See 3 publications below
CoIP	See 1 publications below

Product Information

14155-1-AP targets FKBP5 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FKBP5 fusion protein Ag5337 相同性解析による交差性が予測される生物種
Full Name	FK506 binding protein 5
Calculated molecular weight	457 aa, 51 kDa
Observed molecular weight	51 kDa
GenBank accession number	BC042605
Gene symbol	FKBP5
Gene ID (NCBI)	2289
RRID	AB_2231625
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FKBP5(Peptidyl-prolyl cis-trans isomerase FKBP5 ) interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP. Unlike the predominant T-cell expression of the mouse protein, FKBP5 is expressed as a 51-kD protein in a variety of tissues but not in brain, colon, or lung(PMID: 9125197).

Protocols

Product Specific Protocols
WB protocol for FKBP5 antibody 14155-1-AP	Download protocol
IHC protocol for FKBP5 antibody 14155-1-AP	Download protocol
IP protocol for FKBP5 antibody 14155-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Cell Stem Cell Decoding the temporal and regional specification of microglia in the developing human brain. Authors - Yanxin Li View Article
human	WB	EMBO J USP49 negatively regulates tumorigenesis and chemoresistance through FKBP51-AKT signaling. Authors - Kuntian Luo KD Validated View Article
mouse	IHC	Nutrients Kaempferol, a Phytoprogestin, Induces a Subset of Progesterone-Regulated Genes in the Uterus Authors - Tova M Bergsten View Article
mouse	IHC	Mol Cancer Ther Cell intrinsic role of COX-2 in pancreatic cancer development. Authors - Hill Reginald R View Article
mouse	WB	RNA The co-chaperones Fkbp4/5 control Argonaute2 expression and facilitate RISC assembly. Authors - Martinez Natalia J NJ View Article
mouse	WB	Biochem Pharmacol Developmental nicotine exposure elicits multigenerational disequilibria in proBDNF proteolysis and glucocorticoid signaling in the frontal cortices, striata, and hippocampi of adolescent mice. Authors - Jordan M Buck View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FKBP5 Polyclonal antibody

FKBP5 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis of K-562 using 14155-1-AP

WB analysis of HEK-293 using 14155-1-AP

WB analysis of mouse thymus using 14155-1-AP

WB analysis of mouse thymus using 14155-1-AP

WB analysis of mouse thymus using 14155-1-AP

IP experiment of K-562 using 14155-1-AP

IHC staining of human colon cancer using 14155-1-AP

IHC staining of human colon cancer using 14155-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Decoding the temporal and regional specification of microglia in the developing human brain.

USP49 negatively regulates tumorigenesis and chemoresistance through FKBP51-AKT signaling.

Kaempferol, a Phytoprogestin, Induces a Subset of Progesterone-Regulated Genes in the Uterus

Cell intrinsic role of COX-2 in pancreatic cancer development.

The co-chaperones Fkbp4/5 control Argonaute2 expression and facilitate RISC assembly.

Developmental nicotine exposure elicits multigenerational disequilibria in proBDNF proteolysis and glucocorticoid signaling in the frontal cortices, striata, and hippocampi of adolescent mice.