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- KD/KO Validated
FHL2 Polyclonal antibody
FHL2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, IHC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 21619-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | HeLa cells, U2OS cells, hTERT-RPE1 cells, NIH/3T3 cells |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells, A431 cells |
Planning an IHC experiment? We recommend our IHCeasy FHL2 Ready-To-Use IHC Kit. FHL2 primary antibody included.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 11 publications below |
| IHC | See 3 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
| CoIP | See 4 publications below |
Product Information
21619-1-AP targets FHL2 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FHL2 fusion protein Ag1378 相同性解析による交差性が予測される生物種 |
| Full Name | four and a half LIM domains 2 |
| Calculated molecular weight | 32 kDa |
| Observed molecular weight | 32 kDa |
| GenBank accession number | BC014397 |
| Gene symbol | FHL2 |
| Gene ID (NCBI) | 2274 |
| RRID | AB_10860263 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FHL2 antibody 21619-1-AP | Download protocol |
| IHC protocol for FHL2 antibody 21619-1-AP | Download protocol |
| IF protocol for FHL2 antibody 21619-1-AP | Download protocol |
| IP protocol for FHL2 antibody 21619-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity. | ||
EBioMedicine Up-regulated FHL2 inhibits ovulation through interacting with androgen receptor and ERK1/2 in polycystic ovary syndrome. | ||
Cell Death Dis FHL2 deficiency impairs follicular development and fertility by attenuating EGF/EGFR/YAP signaling in ovarian granulosa cells
| ||
iScience Genetic ablation of diabetes-associated gene Ccdc92 reduces obesity and insulin resistance in mice | ||
Int J Mol Sci FHL2 Inhibits SARS-CoV-2 Replication by Enhancing IFN-β Expression through Regulating IRF-3
| ||
J Virol Structural and Functional Characterization of Host FHL1 Protein Interaction with Hypervariable Domain of Chikungunya Virus nsP3 Protein. |
