Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 16000-1-AP
Human placenta tissue were subjected to SDS PAGE followed by western blot with 16000-1-AP (FGL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16000-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16000-1-AP (FGL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 16000-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16000-1-AP (FGL1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 16000-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 16000-1-AP (FGL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 16000-1-AP
IP result of anti-FGL1 (IP:16000-1-AP, 4ug; Detection:16000-1-AP 1:500) with HeLa cells lysate 2000ug.
IHC staining of human liver cancer using 16000-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16000-1-AP (FGL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 16000-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16000-1-AP (FGL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 16000-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16000-1-AP (FGL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 16000-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16000-1-AP (FGL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human hepatocirrhosis using 16000-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16000-1-AP (FGL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human hepatocirrhosis using 16000-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16000-1-AP (FGL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

FGL1 Polyclonal antibody

FGL1 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 16000-1-AP

Publications(20)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

FGL1, fibrinogen like 1, Fibrinogen like protein 1, Hepassocin, HFREP 1, HFREP1, HP 041, HPS, LFIRE 1, LFIRE1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (20)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human placenta tissue, human liver tissue, HepG2 cells, mouse thymus tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human liver cancer tissue, human hepatocirrhosis tissue, mouse liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 16 publications below
IHC	See 11 publications below
IF	See 2 publications below

Product Information

16000-1-AP targets FGL1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8581 Product name: Recombinant human FGL1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-312 aa of BC007047 Sequence: MAKVFSFILVTTALTMGREISALEDCAQEQMRLRAQVRLLETRVKQQQVKIKQLLQENEVQFLDKGDENTVIDLGSKRQYADCSEIFNDGYKLSGFYKIKPLQSPAEFSVYCDMSDGGGWTVIQRRSDGSENFNRGWKDYENGFGNFVQKHGEYWLGNKNLHFLTTQEDYTLKIDLADFEKNSRYAQYKNFKVGDEKNFYELNIGEYSGTAGDSLAGNFHPEVQWWASHQRMKFSTWDRDHDNYEGNCAEEDQSGWWFNRCHSANLNGVYYSGPYTAKTDNGIVWYTWHGWWYSLKSVVMKIRPNDFIPNVI 相同性解析による交差性が予測される生物種
Full Name	fibrinogen-like 1
Calculated molecular weight	312 aa, 36 kDa
Observed molecular weight	34 kDa, 68 kDa
GenBank accession number	BC007047
Gene Symbol	FGL1
Gene ID (NCBI)	2267
RRID	AB_2103936
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q08830
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FGL1 is a 68 kDa protein that comprises two disulfide linked 34 kDa homodimers. FGL1 is a predominantly liver expressed protein that has been implicated as both a hepatoprotectant and a hepatocyte mitogen. FGL1 expression is decreased in hepatocellular carcinoma (HCC), and it may play a role in the development of hepatocellular carcinomas.

Protocols

Product Specific Protocols
IHC protocol for FGL1 antibody 16000-1-AP	Download protocol
IP protocol for FGL1 antibody 16000-1-AP	Download protocol
WB protocol for FGL1 antibody 16000-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Transl Res Role of placental fibrinogen-like protein 1 in gestational diabetes. Authors - Lin Kang View Article
mouse	IHC	Front Immunol Fibrinogen-like protein 1 promotes liver-resident memory T-cell exhaustion in hepatocellular carcinoma Authors - Changjie Yang View Article
mouse	WB	Diabetologia PMID: 27221093 Authors - Hung-Tsung Wu KD Validated View Article
mouse	WB,IHC	J Nanobiotechnology Regulating the immunosuppressive tumor microenvironment to enhance breast cancer immunotherapy using pH-responsive hybrid membrane-coated nanoparticles. Authors - Chunai Gong View Article
human	WB	Int J Mol Sci Fibrinogen-Like Protein 1 Modulates Sorafenib Resistance in Human Hepatocellular Carcinoma Cells. Authors - Yeonghoon Son View Article
human,mouse	WB	J Clin Endocrinol Metab The Hepatic Protection Effects of Hepassocin in Hyperglycemic Crisis. Authors - Horng-Yih Ou View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16000-1-AP

WB analysis of HepG2 using 16000-1-AP

WB analysis of mouse thymus using 16000-1-AP

WB analysis of human liver using 16000-1-AP

IP experiment of HeLa using 16000-1-AP

IHC staining of human liver cancer using 16000-1-AP

IHC staining of mouse liver using 16000-1-AP

IHC staining of mouse liver using 16000-1-AP

IHC staining of human liver cancer using 16000-1-AP

IHC staining of human hepatocirrhosis using 16000-1-AP

IHC staining of human hepatocirrhosis using 16000-1-AP

FGL1 Polyclonal antibody

FGL1 Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Role of placental fibrinogen-like protein 1 in gestational diabetes.

Fibrinogen-like protein 1 promotes liver-resident memory T-cell exhaustion in hepatocellular carcinoma

PMID: 27221093

Regulating the immunosuppressive tumor microenvironment to enhance breast cancer immunotherapy using pH-responsive hybrid membrane-coated nanoparticles.

Fibrinogen-Like Protein 1 Modulates Sorafenib Resistance in Human Hepatocellular Carcinoma Cells.

The Hepatic Protection Effects of Hepassocin in Hyperglycemic Crisis.