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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FGFR3 Polyclonal antibody

FGFR3 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 55358-1-AP

Synonyms

ACH, CD333, CEK2, FGFR 3, FGFR3, HSFGFR3EX, JTK4

Validation Data Gallery

Applications:
  • IHC staining of human breast cancer using 55358-1-AP

    IHC staining of human breast cancer using 55358-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55358-1-AP (FGFR3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 55358-1-AP

    IHC staining of human colon cancer using 55358-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55358-1-AP (FGFR3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 55358-1-AP

    IHC staining of human lung cancer using 55358-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55358-1-AP (FGFR3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat testis using 55358-1-AP

    IHC staining of rat testis using 55358-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 55358-1-AP (FGFR3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 55358-1-AP

    IHC staining of mouse kidney using 55358-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55358-1-AP (FGFR3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inhuman breast cancer tissue, mouse kidney tissue, rat testis tissue, human lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

55358-1-AP targets FGFR3 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name fibroblast growth factor receptor 3
Calculated molecular weight 88 kDa
GenBank accession numberNM_000142
Gene symbol FGFR3
Gene ID (NCBI) 2261
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fibroblast growth factors (FGFs) are polypeptide growth factors involved in a variety of activities including mitogenesis, angiogenesis, and wound healing (PMID: 1847508). The human FGF receptor family, a subfamily of receptor tyrosine kinases (RTKs), comprises of four family members-FGFR1, FGFR2, FGFR3, and FGFR4 (PMID: 23900974). Each receptor contains an extracellular domain with either two or three immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tyrosine kinase domain. FGFR3 binds acidic and basic fibroblast GH and plays a role in bone development and maintenance. Mutations in the FGFR3 gene lead to craniosynostosis and multiple types of skeletal dysplasia. Due to frequent mutations in certain cancers, the FGFR3 gene has also been associated with tumor progression.

Protocols

Product Specific Protocols
IHC protocol for FGFR3 antibody 55358-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols