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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FES Monoclonal antibody

FES Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1E1B11

Cat no : 67524-1-Ig

Synonyms

feline sarcoma oncogene, FES, FPS, Proto oncogene c Fes, Proto oncogene c Fps

Validation Data Gallery

Applications:
  • WB analysis using 67524-1-Ig

    WB analysis using 67524-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67524-1-Ig (FES antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 67524-1-Ig

    IF Staining of A549 using 67524-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using 67524-1-Ig (FES antibody), at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inTHP-1 cells, TF-1 cells, HL-60 cells, U-937 cells
Positive IF detected inA549 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:150-1:600
Sample-dependent, check data in validation data gallery

Product Information

67524-1-Ig targets FES in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FES fusion protein Ag28108 相同性解析による交差性が予測される生物種
Full Name feline sarcoma oncogene
Calculated molecular weight 822 aa, 94 kDa
Observed molecular weight 93 kDa, 79 kDa, 87 kDa, 85 kDa
GenBank accession numberBC035357
Gene symbol FES
Gene ID (NCBI) 2242
RRIDAB_2882744
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FES antibody 67524-1-IgDownload protocol
IF protocol for FES antibody 67524-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols