Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 12592-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12592-1-AP (FDX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12592-1-AP
WB result of FDX1 antibody (12592-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FDX1 transfected HEK-293 cells.
WB analysis of HepG2 using 12592-1-AP
WB result of FDX1 antibody (12592-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FDX1 transfected HepG2 cells.
WB analysis using 12592-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12592-1-AP (FDX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 12592-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12592-1-AP (FDX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 12592-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12592-1-AP (FDX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 12592-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12592-1-AP (FDX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 12592-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12592-1-AP (FDX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 12592-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12592-1-AP (FDX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 12592-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12592-1-AP (FDX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 12592-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FDX1 antibody (12592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Featured Product
KD/KO Validated

FDX1 Polyclonal antibody

FDX1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12592-1-AP

Publications(96)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

ADX, FDX, Ferredoxin-1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (96)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, HEK-293 cells, HepG2 cells, PC-3 cells, mouse testis tissue
Positive IHC detected in	human kidney tissue, human liver tissue, human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 9 publications below
WB	See 83 publications below
IHC	See 37 publications below
IF	See 17 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

12592-1-AP targets FDX1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3301 Product name: Recombinant human FDX1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-184 aa of BC017063 Sequence: MAAAGGARLLRAASAVLGGPAGRWLHHAGSRAGSSGLLRNRGPGGSAEASRSLSVSARARSSSEDKITVHFINRDGETLTTKGKVGDSLLDVVVENNLDIDGFGACEGTLACSTCHLIFEDHIYEKLDAITDEENDMLDLAYGLTDRSRLGCQICLTKSMDNMTVRVPETVADARQSIDVGKTS 相同性解析による交差性が予測される生物種
Full Name	ferredoxin 1
Calculated molecular weight	184 aa, 19 kDa
Observed molecular weight	14 kDa
GenBank accession number	BC017063
Gene Symbol	FDX1
Gene ID (NCBI)	2230
RRID	AB_11182486
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P10109
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FDX1(Ferredoxin-1) is also named as ADX, FDX, YAH1, LOH11CR1D and belongs to the adrenodoxin/putidaredoxin family. It is a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450 and it can only reduce mitochondrial CYP enzymes that are essential in adrenal steroidogenesis, bile acid formation, and vitamin D synthesis. The full length has a transit peptide of 60 amino acids.

Protocols

Product Specific Protocols
IF protocol for FDX1 antibody 12592-1-AP	Download protocol
IHC protocol for FDX1 antibody 12592-1-AP	Download protocol
WB protocol for FDX1 antibody 12592-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells. Authors - Ying Wang View Article
human	WB	Mol Cell p53 induces circFRMD4A to suppress cancer development through glycolytic reprogramming and cuproptosis Authors - Quan Liao View Article
human	WB	Mol Cell Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation. Authors - Ross A Weber View Article
human	WB,IHC,IF	Adv Sci (Weinh) High-Efficiency Carriers' Separation Strategy Based Ultrasmall-Bandgap CuWO4 Sono-Enhances GSH Antagonism for Cuproptosis Cascade Immunotherapy Authors - Lichao Zhu View Article
human	WB	Nat Chem Biol Mitochondrial metabolism promotes adaptation to proteotoxic stress. Authors - Peter Tsvetkov KO Validated View Article
mouse	WB,IHC	J Immunother Cancer C5a/C5aR pathway blocking promoted CuS-mediated cancer therapy effect by inhibiting cuproptosis resistance Authors - Hong Yang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 12592-1-AP

WB analysis of HEK-293 using 12592-1-AP

WB analysis of HepG2 using 12592-1-AP

WB analysis using 12592-1-AP

IHC staining of human kidney using 12592-1-AP

IHC staining of human kidney using 12592-1-AP

IHC staining of human liver using 12592-1-AP

IHC staining of human liver using 12592-1-AP

IHC staining of human ovary tumor using 12592-1-AP

IHC staining of human ovary tumor using 12592-1-AP

IF Staining of HepG2 using 12592-1-AP

FDX1 Polyclonal antibody

FDX1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.

p53 induces circFRMD4A to suppress cancer development through glycolytic reprogramming and cuproptosis

Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation.

High-Efficiency Carriers' Separation Strategy Based Ultrasmall-Bandgap CuWO4 Sono-Enhances GSH Antagonism for Cuproptosis Cascade Immunotherapy

Mitochondrial metabolism promotes adaptation to proteotoxic stress.

C5a/C5aR pathway blocking promoted CuS-mediated cancer therapy effect by inhibiting cuproptosis resistance