FBXO41 Polyclonal antibody
FBXO41 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 24519-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 2 publications below |
Product Information
The immunogen of 24519-1-AP is FBXO41 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FBXO41 fusion protein Ag21595 相同性解析による交差性が予測される生物種 |
| Full Name | F-box protein 41 |
| Calculated molecular weight | 875 aa, 94 kDa |
| Observed molecular weight | 100 kDa |
| GenBank accession number | BC112202 |
| Gene symbol | FBXO41 |
| Gene ID (NCBI) | 150726 |
| RRID | AB_2879586 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FBXO41 also named as F box only protein 41 is a 875 amino-acid protein, which directly interact with SKP1 and CUL1. FBXO41 is identified as a Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex, which as a regulator mediates some protein ubiquitination degradation. FBXO41 consists of F-box domain and has zinc ion binding activity. FBXO41 may have an association with Parkinson's disease.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FBXO41 antibody 24519-1-AP | Download protocol |
| IHC protocol for FBXO41 antibody 24519-1-AP | Download protocol |
| IP protocol for FBXO41 antibody 24519-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1. | ||
iScience Neuronal F-Box protein FBXO41 regulates synaptic transmission and hippocampal network maturation. |
