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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FBXL15 Polyclonal antibody

FBXL15 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 20895-1-AP

Synonyms

F box only protein 37, F box/LRR repeat protein 15, Fbl15, FBXL15, FBXO37, JET, PSD

Validation Data Gallery

Applications:
  • WB analysis of MDA-MB-453s using 20895-1-AP

    WB analysis of MDA-MB-453s using 20895-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 20895-1-AP (FBXL15 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • IHC staining of human breast cancer using 20895-1-AP

    IHC staining of human breast cancer using 20895-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20895-1-AP (FBXL15 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 20895-1-AP

    IHC staining of human breast cancer using 20895-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20895-1-AP (FBXL15 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inMDA-MB-453s cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20895-1-AP is FBXL15 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXL15 fusion protein Ag14949 相同性解析による交差性が予測される生物種
Full Name F-box and leucine-rich repeat protein 15
Calculated molecular weight 300 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC002912
Gene symbol FBXL15
Gene ID (NCBI) 79176
RRIDAB_2878758
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FBXL15 antibody 20895-1-APDownload protocol
IHC protocol for FBXL15 antibody 20895-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols