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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FAH Monoclonal antibody

FAH Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rat, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2F8E11

Cat no : 67986-1-Ig

Synonyms

Beta diketonase, FAA, FAH, Fumarylacetoacetase, Fumarylacetoacetate hydrolase

Validation Data Gallery

Applications:
  • WB analysis using 67986-1-Ig

    WB analysis using 67986-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67986-1-Ig (FAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of pig liver using 67986-1-Ig

    WB analysis of pig liver using 67986-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67986-1-Ig (FAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat liver using 67986-1-Ig

    WB analysis of rat liver using 67986-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67986-1-Ig (FAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 67986-1-Ig

    WB analysis of mouse liver using 67986-1-Ig

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 67986-1-Ig (FAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 67986-1-Ig

    IF Staining of HepG2 using 67986-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using FAH antibody (67986-1-Ig, Clone: 2F8E11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inLNCaP cells, pig liver tissue, rat liver tissue, mouse liver tissue, HepG2 cells, Jurkat cells, K-562 cells
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67986-1-Ig targets FAH in WB, IF, ELISA applications and shows reactivity with Human, pig, rat, mouse samples.

Tested Reactivity Human, pig, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FAH fusion protein Ag6704 相同性解析による交差性が予測される生物種
Full Name fumarylacetoacetate hydrolase (fumarylacetoacetase)
Calculated molecular weight 46 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC002527
Gene symbol FAH
Gene ID (NCBI) 2184
RRIDAB_2918735
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAH is also named as FAA(fumarylacetoacetase), the last enzyme in tyrosine degradation, functionally important in the liver and kidney(PMID:8473520). FAH is a metabolic enzyme catalyzing the last step of tyrosine and phenylalanine catabolism: the hydrolysis of fumarylacetoacetate into acetoacetate and fumarate. In humans, deficiency of this activity is associated with the metabolic disease hereditary tyrosinaemia type I, which is also known as hepatorenal tyrosinaemia(PMID:17768357).

Protocols

Product Specific Protocols
WB protocol for FAH antibody 67986-1-IgDownload protocol
IF protocol for FAH antibody 67986-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols